Sequences from confirmation amplicon


Confirmation sequences for line 038G08 and hit At3g14225 are shown.
Line ID 038G08
Locus At3g14225 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4735430
Primers used for PCR 11nh - 8474, show primer details
   
Sequence Name 27-K114405-0022-038-G08C-11nh
GenBank Accession LN498497 [GenBank]
Sequencing primer gene specific primer, 11nh: 5'-AGCGTTTTCGCATAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114405-0022-038-G08C-11nh
ATTCCCCTCACGGCCCACTCCCGCAACATGCCATCTCCCCTTCCTTAAACCGTAACGAGA
AGGATTGTTGATTCTTTGGGATAGAGAAGTGTGAAAGTCATGAAGAGCGTATTTGAATCC
GGATAACCGGCGCTCTAGACGCCTCAAGACTTTTGGAAATTCTTGGTTATGGAGATTGAT
CAGCTCTGTGACTGGCTCGAAACAAGATCCTATCTTTGTACTGTTTATTATCAATGCGCT
TGGTGTACAACCAAATGGACCCAAGCTCAAGAATCCGAATTTCCTTGCTCCTAATTTATA
CAACTCCTGAAAAGAAAGATTTCACATTTTTATCATACATAATCAAAGGTTCTATTCCTC
TCTGCTTTTAGTTGACAAAACCAGTTACTTACCTCGATTACAGTCGTTGTGTTACCGATG
ACGAAATCGATGAATCTTTCTTTGGTAGTGTTACTAAAAGTTGAGGTATTTGCAAAGAAT
GGGTATTGATAGTCATTTGCTCCTATGTGAAAAAGATAAACAGCTTTCGAGATTACTCTC
CTCGCCTCTGCATCTCCTAAATTAGACCTCAACGTTTTCTCCACGTTCTTGAAGTTGTTT
AACTGTGTTCCCAAATCTTTCGACTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCANAAATGTAGATGTCCGCA
   
Sequence Name 05-K114404-0022-038-G08C-8409
GenBank Accession LN488449 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114404-0022-038-G08C-8409
TCGGATGAGCATTTCAAGTCGAAAGATTTGGGAACACAGTTAACAACTTCAAGAACGTGG
AGAAAACGTTGAGGTCTAATTTAGGAGATGCAGAGGCGAGGAGAGTAATCTCGAAAGCTG
TTTATCTTTTTCACATAGGAGCAAATGACTATCAATACCCATTCTTTGCAAATACCTCAA
CTTTTAGTAACACTACCAAAGAAAGATTCATCGATTTCGTCATCGGTAACACAACGACTG
TAATCGAGGTAAGTAACTGGTTTTGTCAACTAAAAGCAGAGAGGAATAGAACCTTTGATT
ATGTATGATAAAAATGTGAAATCTTTCTTTTCAGGAGTTGTATAAATTAGGAGCAAGGAA
ATTCGGATTCTTGAGCTTGGGTCCATTTGGTTGTACACCAAGCGCATTGATAATAAACAG
TACAAAGATAGGATCTTGTTTCGAGCCAGTCACAGAGCTGATCAATCTCCATAACCAAGA
ATTTCCAAAAGTCTTGAGGCGTCTAGAGCGCCGGTTATCCGGATTCAAATACGCTCTTCA
TGACTTTCACACTTCTCTATCCCAAAGAATCAACAATCCTTCTCGTTACGGTTTTAAGGA
AGGGGAGATGGCATGTTGCGGGAGTGGGCCGTTGAGGGGAATCAATACATGTGGATTCCG
AAATGGACCATCGCANGGTTACAAGCTA

Last Updated on 06.12.2018 16:11