Sequences from confirmation amplicon


Confirmation sequences for line 038G12 and hit At5g50550 are shown.
Line ID 038G12
Locus At5g50550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20578222
Primers used for PCR 49mv - 8474, show primer details
   
Sequence Name 59-K114263-0022-038-G12C-49mv
GenBank Accession LN510062 [GenBank]
Sequencing primer gene specific primer, 49mv: 5'-CACATGCATCAGTTAAAAGCCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K114263-0022-038-G12C-49mv
AATGATTGTAATAAATTTCTTGATAGTGATATTTTTCTGTTTGCAGCGAAAGTGGTAAGT
TTCTTGTATCTCTTGGAGCTCCACTTTGTCGGGTTTGGGATGTGAATGCTTCTGCTGCTA
TTGCCAGTCTATCAAAAGAGAAGGTCTGTTGGGTTTTGCTCTTTACTAGTTTCTCTTAAC
TTTATTGTTGTATTGGATCTGATGAGAGTTTGAATACTGAAATGCAGGACGAGATGTTTG
CCTCGTGTAGATTCTCTGTAGACAATTCAGGCAGTGAAGTTCTTTATGTTGCTGCAAACA
CTCGTAACTCATATTTATTCTCACAGTGCAAGAACATAAGAACCCTTTTTGTCTCAGCCA
ACAATTGAGAGATTTGATTTGTTTTGTGTAGAACGTGGTGGGAGTATTATAACATGGGAT
ACAACATCATGGAGAAGAAGAAGTTCAAAGCTTATTAAAAGAAACAACTCTNTATCTGCC
TTTAATGTCTCAGCTGATGGGAAGCTTCTTGCAGTGTAAGTTTATCTTTCTTCTCACTTA
TGGGGCCAAACAGAAATACTATATTTTGTTTTCATGTTTTTTTCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
   
Sequence Name 16-K114262-0022-038-G12C-8409
GenBank Accession LN493520 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114262-0022-038-G12C-8409
TATATCCTGAAAAAACATGAAAACAAAATATAGTATTTCTGTTTGGCCCCATAAGTGAGA
AGAAAGATAAACTTACACTGCAAGAAGCTTCCCATCAGCTGAGACATTAAAGGCAGATAT
AGAGTTGTTTCTTTTAATAAGCTTTGAACTTCTTCTTCTCCATGATGTTGTATCCCATGT
TATAATACTCCCACCACGTTCTACACAAAACAAATCAAATCTCTCAATTGTTGGCTGAGA
CAAAAAGGGTTCTTATGTTCTTGCACTGTGAGAATAAATATGAGTTACGAGTGTTTGCAG
CAACATAAAGAACTTCACTGCCTGAATTGTCTACAGAGAATCTACACGAGGCAAACATCT
CGTCCTGCATTTCAGTATTCAAACTCTCATCAGATCCAATACAACAATAAAGTTAAGAGA
AACTAGTAAAGAGCAAAACCCAACAGACCTTCTCTTTTGATAGACTGGCAATAGCAGCAG
AAGCATTCACATCCCAAACCCGACNAAGTGGAGCTCCNAGAGATACAAGAAACTTACCAC
TTTCGCTGCAAACAGAANAATATCACTATCAAGAAATTTATTTACAATCATTTCTCCNAA
GATGATCCCTTTCTCACCTGANAGTTAGGCTTTTAA

Last Updated on 06.12.2018 16:11