Sequences from confirmation amplicon


Confirmation sequences for line 039B02 and hit At4g21210 are shown.
Line ID 039B02
Locus At4g21210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11307865
Primers used for PCR MM26 - 8474, show primer details
   
Sequence Name 29-K019778-022-039-B02C-MM26
GenBank Accession LN499094 [GenBank]
Sequencing primer gene specific primer, MM26: 5'-AAACAAAGTCTTGGGAAGATCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K019778-022-039-B02C-MM26
CAATCGGTACGTTAGAGACTTTGTAGCCCTTTTGAGCTAAGTAAGTCGAGAGTGGTGTCT
TCCCTGTCCTAGAGACACCTACAAGAACAATGTCAGCTTTTTCAAGATTCTCGGGCAAGG
CACCGTCGTCGTGTTTGATAGTGAATTCGATAGCTTCGATTCTTTTGAAGTAGTCTTCGT
TGAGAGATGAGTTTGTTATGCCACGTGAAAGACCGGAAGGATTAGTGCCTAAGTGAGAGG
AGATTGACTCTGTGATTGGTCCAAGAATGTCTAATGATGGGATTTTCCATAGCTTGCAAG
CTCTCATGGTTGCTTCATGTCATTGATGGAGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACATTCA
   
Sequence Name 30-K019778-022-039-B02C-8409
GenBank Accession LN499531 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K019778-022-039-B02C-8409
CATGTAGATTTCTCCATCAATGACATGAAGCAACCATGAGAGCTTGCAAGCTATGGAAAA
TCCCATCATTAGACATTCTTGGACCAATCACAGAGTCAATCTCCTCTCACTTAGGCACTA
ATCCTTCCGGTCTTTCACGTGGCATAACAAACTCATCTCTCAACGAAGACTACTTCAAAA
GAATCGAAGCTATCGAATTCACTATCAAACACGACGACGGTGCCTTGCCCGAGAATCTTG
AAAAAGCTGACATTGTTCTTGTAGGTGTCTCTAGGACAGGGAAGACACCACTCTCGACTT
ACTTAGCTCAAAAGGGCTACAAAGTCTCTAACGTACCGATTGTGAACGGTGTTGATCTTC
CCAAGACTTTGT
Primers used for PCR FL81 - 3144, show primer details
   
Sequence Name 01-K035345-022-039-B02C-8409
GenBank Accession LN486475 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K035345-022-039-B02C-8409
ATTTCTGCCATTGATGGATCACCTAAAGTGTAGATTACCCTTGCTCCTTCTCTTGCCGCT
TGCTTTATGATCTCCATTAACTTCTCCCCATCTTCAATCTTCAAAAAACCAAATAACCAA
TCAAGTAAACTAACTCAATTCCGGTTTAAGATAACCGGAATCAATTATTCCGGTTCAAGA
AAACAGAAATAATTTCGGTCCGATTCACAACGGCTAAATTGAATTACAGACATTTTCAGC
TGAAATTAGTAGGAATTGTCATTTGGTTGGATTCGGTTTAAAGTTCGATTTCAACAATTA
AGGAAATTTAAATTGTAAACCGAATTCATTCCCGTTTGGTTTGGTTTAGTAAAAA
   
Sequence Name 49-K035345-022-039-B02C-FL81
GenBank Accession LN506754 [GenBank]
Sequencing primer gene specific primer, FL81: 5'-TTTTTACTAAACCAAACCAAACGG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K035345-022-039-B02C-FL81
TGAAATCGAACTTTAAACCGAATCCAACCAAATGACAATTCCTACTAATTTCAGCTGAAA
ATGTCTGTAATTCAATTTAGCCGTTGTGAATCGGACCGAAATTATTTCTGTTTTCTTGAA
CCGGAATAATTGATTCCGGTTATCTTAAACCGGAATTGAGTTAGTTTACTTGATTGGTTA
TTTGGTTTTTTGAAGATTGAAGATGGGGAGAAGTTAATGGAGATCATAAAGCAAGCGGCA
AGAGAAGGAGCAATGGTAATCTACACTTTAGCTGATCCATCAATGGCAGAAATCCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
TCACTCCCAAAT

Last Updated on 06.12.2018 16:11