Sequences from confirmation amplicon


Confirmation sequences for line 039E09 and hit At4g23460 are shown.
Line ID 039E09
Locus At4g23460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12248823
Primers used for PCR EV45 - 8474, show primer details
   
Sequence Name 23-K037447-022-039-E09C-EV45
GenBank Accession LN496470 [GenBank]
Sequencing primer gene specific primer, EV45: 5'-ATACTGTGGGTAAGATCGGTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K037447-022-039-E09C-EV45
GCTCATCTTCTCCATATCCTGTTTCGGCATTTTCACAAGGATCTGATTTCGACGACGAAA
TGAGGTCAAGCGAGAGAGAAGCGATTTAAGGATCAGAGACTCTTCGCGAAAAGTCTTTGA
GAATCTGATCCACAATCACATCTAAAAGCTGTTCTCTCACAAACGTGTTTCTGACTTCAT
CTCTCTCATATCGACAACTTGAGAATACAAATTCTTCTTCTCCCCTCCCCTGGATGGCAA
CTGGCGTTGACTAGACGAAGTCACGAGGCAATTTCGTCAAATATCAATAATACTAGGGTT
AAACTGTAATTAGTTTTATTTTAACCCCGACCTATTACTTTTTGCAATTTCGTGTCTTTG
TGTCTCTTGTCACTGATCGTCTCGGAGGAGAAGAAGTGTGGAGGTTGAAGAAGGGAGAAA
ATGAGCGGTCATGATTCTAAATATTTTTCGACTACGAAGAAGGGAGAGATCCCTGAGCTC
AAGGAAGAGCTGAATTAGAATATTTTTGAATGGCGGTCATGATTCTAAATATTCAATTGA
AGCCATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTN
TATNTCGACGTGTCTACTCCCCCCCAAAAGA
   
Sequence Name 31-K037447-022-039-E09C-8409
GenBank Accession LN500073 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K037447-022-039-E09C-8409
GAGCCATTTCAATTGAATTACATGGCTTCAATTGAATATTTAGAATCATGACCGCCATTC
AAAAATATTCTAATTCAGCTCTTCCTTGAGCTCAGGGATCTCTCCCTTCTTCGTAGTCGA
AAAATATTTAGAATCATGACCGCTCATTTTCTCCCTTCTTCAACCTCCACACTTCTTCTC
CTCCGAGACGATCAGTGACAAGAGACACAAAGACACGAAATTGCAAAAAGTAATAGGTCG
GGGTTAAAATAAAACTAATTACAGTTTAACCCTAGTATTATTGATATTTGACGAAATTGC
CTCGTGACTTCGTCTAGTCAACGCCAGTTGCCATCCAGGGGAGGGGAGAAGAAGAATTTG
TATTCTCAAGTTGTCGATATGAGAGAGATGAAGTCAGAAACACGTTTGTGAGAGAACAGC
TTTTAGATGTGATTGTGGATCAGATTCTCAAAGACTTTTCGCGAAGAGTCTCTGATCCTT
AAATCGCTTCTCTCTCGCTTGACCTCATTTCGTCGTCGAAATCAGATCCTTGTGAAAATG
CCGAAACAGGATATGGAGAAGATGGAGCTTCGTAAGAACTTCCGTAATGTTTGGCATACC
GAT

Last Updated on 06.12.2018 16:11