Sequences from confirmation amplicon


Confirmation sequences for line 039F11 and hit At5g66320 are shown.
Line ID 039F11
Locus At5g66320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26496232
Primers used for PCR 01uj - 8474, show primer details
   
Sequence Name 46-K117452-0022-039-F11C-01uj
GenBank Accession LN505990 [GenBank]
Sequencing primer gene specific primer, 01uj: 5'-GGTGTCACTTTTAGTTTGGTCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117452-0022-039-F11C-01uj
AATCAAAGCTATAAACCAATTCGAACGGTTTCAAATTAACTCCATATTTCCCATTATGTA
ACGTATCTATATGTAAACAAACTTTAAGCTATAACGCAGAACTTTGAAATGGGGAAATTT
AAACTGAAAAAATTAACGCGAAAGAGTACAATACAAACGAGTTTTTGACCTAATCAGTTA
CTAAGAACTTACGGATACTCCTAGCATGAAAAATCAAACTACTGTAACAAAAAAAAAGTA
AATGCTAAAAATAATGAAAGTAATATACAAAGAAACAAGTCAATGAGAAACTAACTTGGG
GCTAACAACTGAACCGGTTCGGATCCTAAATCTCTGCTCTAATTAAGGCCCTATTTCTTC
AAAAACTTGGTACAGCTTGTGGGGACCAAGCTGATCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 23-K117451-0022-039-F11C-8409
GenBank Accession LN496727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117451-0022-039-F11C-8409
CCCACAAGCGGNTACCAAGNTTTTTGAAGAAATAGGGCCTTAATTAGAGCAGAGATTTAG
GATCCGAACCGGTTCAGTTGTTAGCCCCAAGTTAGTTTCTCATTGACTTGTTTCTTTGTA
TATTACTTTCATTATTTTTAGCATTTACTTTTTTTTTGTTACAGTAGTTTGATTTTTCAT
GCTAGGAGTATCCGTAAGTTCTTAGTAACTGATTAGGTCAAAAACTCGTTTGTATTGTAC
TCTTTCGCGTTAATTTTTTCAGTTTAAATTTCCCCATTTCAAAGTTCTGCGTTATAGCTT
AAAGTTTGTTTACATATAGATACGTTACATAATGGGAAATATGGAGTTAATTTGAAACCG
TTCGAATTGGTTTATAGCTTTGATTTTAGGAACGCTACAAACCATCGCATTAATGAATGG
ACCAAA

Last Updated on 06.12.2018 16:11