Sequences from confirmation amplicon


Confirmation sequences for line 040B01 and hit At5g46130 are shown.
Line ID 040B01
Locus At5g46130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18703984
Primers used for PCR 43eq - 8474, show primer details
   
Sequence Name 17-K110713-0022-040-B01C-43eq
GenBank Accession LN493900 [GenBank]
Sequencing primer gene specific primer, 43eq: 5'-TCACCATGTCCAAGAAAAATACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K110713-0022-040-B01C-43eq
TATACATATCTTTGTGTCTTTAGCATTTTCTTTCTCTTTAACACCAAGAACCGCTTTGTT
TTGTGCACGACTGTGTCTGACTCAAAGGCGTCCCCATAGTACCTTTGAACACATGAGAGA
GCCATTAATGAATCAGCTAATAGGTGTTTACTTATAAAAGAAAAGAAAAAGAAAGTATAT
ATGTAGTGTACCATGAGATGAGGAATTGTTCCCCGGAGGGTGACTCCACTAGATGATCTG
TCCTGGTAAACGAATTCATCTCCTCCAACTCATACAACATATACTGAGGAAGATCTTTTT
TGTCAATTATATGATACGTGGGCTCGTCTTTCTCTTTGAAGTTGAGACCAAATGAGCACA
TCCAATCGCCTCCAGGAGTTGAAGTGTAAAATCTTTGCTCTTTCTTAGAGTACTTGAGAC
TTGAGTATGGGCATATACACTCATGCCCATGCGTGGTTTCGATATTGATCCACCGTAAGT
CTTTATGCCTATAGAGCATAAGCTGAGATACCGAAAACTTGATAGCAACGACCCAATCAT
CATTTTTTAGGTCAGGAAGAGAGGTCATGGCAATGTTTTGGATTTGAGAGCCTTTTGGAA
GAGGAGGTAGATGAACAGAAACAGATTTTGATTTAGGGTCAGAGGGCTTGTAATAGATAT
TTAGACGAACGTTGTCCATATCACGGAGGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 01-K110711-0022-040-B01C-8409
GenBank Accession LN486624 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K110711-0022-040-B01C-8409
ATTTACATTGAATATATCCCTCCGTGATATGGACAACGTTCGTCTAAATCTCTATTACAA
GCCCTCTGACCCTAAATCAAAATCTGTTTCTGTTCATCTACCTCCTCTTCCAAAAGGCTC
TCAAATCCAAAACATTGCCATGACCTCTCTTCCTGACCTAAAAAATGATGATTGGGTCGT
TGCTATCAAGTTTTCGGTATCTCAGCTTATGCTCTATAGGCATAAAGACTTACGGTGGAT
CAATATCGAAACCACGCATGGGCATGAGTGTATATGCCCATACTCAAGTCTCAAGTACTC
TAAGAAAGAGCAAAGATTTTACACTTCAACTCCTGGAGGCGATTGGATGTGCTCATTTGG
TCTCAACTTCAAAGAGAAAGACGAGCCCACGTATCATATAATTGACAAAAAAGATCTTCC
TCAGTATATGTTGTATGAGTTGGAGGAGATGAATTCGTTTACCAGGACAGATCATCTAGT
GGAGTCACCCTCCGGGGAACAATTCCTCATCTCATGGTACACTACATATATACTTTCTTT
TTCTTTTCTTTTATAAGTAAACACCTATTAGCTGATTCATTAATGGCTCTCTCATGTGTT
CAAAGGTACTATGGGGACGCCTTTGAGTCAGACACAGTCGTGCACAAAACAAAGCGGTTC
TTGGTGTTTAAAGAGAAAGAAAATGCTAAAAGACACAAAGATATGTATTACACTGAAAAT
ATTGGAGATCTTTGTATTTTTCTGGAACATGGTGA

Last Updated on 06.12.2018 16:11