Sequences from confirmation amplicon


Confirmation sequences for line 040B02 and hit At1g15280 are shown.
Line ID 040B02
Locus At1g15280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5253583
Primers used for PCR 03dl - 3144, show primer details
   
Sequence Name 23-K110124-0022-040-B02C-03dl
GenBank Accession LN496599 [GenBank]
Sequencing primer gene specific primer, 03dl: 5'-AGGGTTGGATTTTTCAAAGTAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K110124-0022-040-B02C-03dl
AGGAGAGATAATTGACTCTAAGGTGGAGTTATTGACCTCTTATTATCTTGCAATTTTATC
AAGCATGAGGGGTTTGAGTAGCTGCATTGGGGATTGCTTAGTTCATACTATTGGGAAATA
TGATTTTTTCTGAATATAGCTCTTCCTCTCTACCTTCTATCTGTCGATTGATATAGTTCT
TTATCTTGCTTTCGTAGGCGAATGCGTGGTGGGAGAAGACAGTGGGGCTCTGGAGAGGAA
AGAAAATGGGGACATGACAAATTTGAAGAGATGAATACTGGGGAAAAACATTCTGATGTA
AAGCTTCTGTCTATGGTTTTTAGAATAATTTTAGATTCCTCTTATCTCTTAATTGCTATA
ACATGTACATGTGACATATGTTAGCTTTGTTGTATTTATGCACTTTCCGTCTCTTATCTT
TTTCCTTTCTTTCTTAAACTGCATTTGATACACAGCAGAGGATGTCTAGAGGCCGATTCA
GAGGTCATGGTCGAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCC
GGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAA
GGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAC
   
Sequence Name 07-K110123-0022-040-B02C-3144
GenBank Accession LN489320 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K110123-0022-040-B02C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTCGAC
CATGACCTCTGAATCGGCCTCTAGACATCCTCTGCTGTGTATCAAATGCAGTTTAAGAAA
GAAAGGAAAAAGATAAGAGACGGAAAGTGCATAAATACAACAAAGCTAACATATGTCACA
TGTACATGTTATAGCAATTAAGAGATAAGAGGAATCTAAAATTATTCTAAAAACCATAGA
CAGAAGCTTTACATCAGAATGTTTTTCCCCAGTATTCATCTCTTCAAATTTGTCATGTCC
CCATTTTCTTTCCTCTCCAGAGCCCCACTGTCTTCTCCCACCACGCATTCGCCTACGAAA
GCAAGATAAAGAACTATATCAATCGACAGATAGAAGGTAGAGAGGAAGAGCTATATTCAG
AAAAAATCATATTTCCCAATAGTATGAACTAAGCAATCCCCAATGCAGCTACTCAAACCC
CTCATGCTTGATAAAATTGCAAGATAATAAGAGGTCAATAACTCCACCTTAGAGTCAATT
ATCTCTCCTCTAAATACAATTTCATCCTAAGTTAATACCTACTTTGAAA

Last Updated on 06.12.2018 16:11