Sequences from confirmation amplicon


Confirmation sequences for line 040C09 and hit At3g60400 are shown.
Line ID 040C09
Locus At3g60400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22330634
Primers used for PCR NB56 - 8474, show primer details
   
Sequence Name 15-K101267-0022-040-C09C-8409
GenBank Accession LN492902 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K101267-0022-040-C09C-8409
TTGAATAATTCATCGCAATTGCTCATTTCTCGCTAACCCTTTCTCCACAAGCCACTTATG
GAATCTAAACCTTGGACTGATTCTGTTCTCCAGGTCAAAACAAAGATAAGCTGGGAAAAC
CTCTAGGTAATCCAAAGAATCTCCCATCTCGCCACAGAGGAACCTTAGTTTATCTTGTAT
GCTATGTGGTTTCTGATTCAAGATCTTTGGTGCTGATCTTATCAACATGCATATCTTTGA
GAAAATGATGCCACTGTTTAGTAAGATTTGGAATCGGTCGTGGAGTTCTACCGCCGTACC
ATGTACATGTTGTAGTACCTTCATGGTGATGCCGTTTTCGCCAAACCCTATTTCATGAAG
GAAATCCAACTTTTGAATGTTGTGTCTTTTGGTTCTTGAGTTTTGAAGCTCCTCCAAGCC
TTCTTGATACTCTCTCTCTAAGTCTTCATCGGGATCCATCAAGGTATAGCTAGCGAGTAA
ATGATGGTTCCCATTCTTCAATATATCAAAGATCCTTTCATGCAAATCTATGGCTCTNAG
CACATA
   
Sequence Name 15-K101273-0022-040-C09C-NB56
GenBank Accession LN492903 [GenBank]
Sequencing primer gene specific primer, NB56: 5'-ATTGAAGAATCTGCCTTATGTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K101273-0022-040-C09C-NB56
CTTTGATATATTGAAGAATGGGAACCATCATTTACTCGCTAGCTATCCCTTGATGGATCC
CGATGAAGACTTAGAGAGAGAGTATCACGAAGGCTTGGAGGAGCTTCAAAACTCAAGAAC
CAAAAGACACAACATTCAAAAGTTGGATTTCCTTCATGAAATAGGGTTTGGCGAAAACGG
CATCACCATGAAGGTACTACAACATGTACATGGTACGGCGGTAGAACTCCACGACCGATT
CCAAATCTTACTAAACAGTGGCATCATTTTCTCAAAGATATGCATGTTGATAAGATCAGC
ACCAAAGATCTTGAATCAGAAACCACATAGCATACAAGATAAACTAAGGTTCCTCTGTGG
CGAGATGGGAGATTCTTTGGATTACCTAGAGGTTTTCCCAGCTTATCTTTGTTTTGACCT
GGAGAACAGAATCAGTCCAAGGTTTAGATTCCATAAGTGGCTTGTGGAGAAAGGGTTTAG
CGAGAAATGAGCAATTGCGATGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATATCCGCA

Last Updated on 06.12.2018 16:11