Sequences from confirmation amplicon


Confirmation sequences for line 040D01 and hit At5g16320 are shown.
Line ID 040D01
Locus At5g16320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5344522
Primers used for PCR 06kk - 8474, show primer details
   
Sequence Name 61-K113595-0022-040-D01C-06kk
GenBank Accession LN510573 [GenBank]
Sequencing primer gene specific primer, 06kk: 5'-AAATCAGATTCTACAGGAAGCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113595-0022-040-D01C-06kk
TGGGTCTGAATTAGAATTTTCCTCTCAAATTCGCGCTTATCTATCTCCTCCCATCGATTT
CATCGTCTTCACGCTAGCTGCAAACTCGAAAGAGAGACTATAGACTGGTGTGGTCTCGAT
TTTGTCTTAATAGAGGTCGCTCTAAACCGAGTAGAAACGAAATTGAATTCATCCAAACCA
TTTCAATTTCTATAAATCGAACCAAATCAAGTGAAAAACAATTCGGTTCTGGTTTTATTA
AACTTACTCGAACCGAACCGAACCGATNACGTTCTTATATATCCTTGCCGGAGAGAGCCA
AAGAAATCTTAGAGATCAAACAATGACGGCGAGTGAGAAAAAGAAAGAGTAATTACCAAT
TTTAAATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K113594-0022-040-D01C-8409
GenBank Accession LN494841 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K113594-0022-040-D01C-8409
TCATTGAATATATCCATTTAAAATTGGTAATTACTCTTTCTTTTTCTCACTCGCCGTCAT
TGTTTGATCTCTAAGATTTCTTTGGCTCTCTCCGGCAAGGATATATAAGAACGTTATCGG
TTCGGTTCGGTTCGAGTAAGTTTAATAAAACCAGAACCGAATTGTTTTTCACTTGATTTG
GTTCGATTTATAGAAATTGAAATGGTTTGGATGAATTCAATTTCGTTTCTACTCGGTTTA
GAGCGACCTCTATTAAGACAAAATCGAGACCACACCAGTCTATAGTCTCTCTTTCGAGTC
TGCAGCTAGCGTGAAGACGATGAAATCGATGGGAGGAGATAGATAAGCGCGAATTTGAGA
GTGAAAATTCTAAATTCAGACCCATTTTCACCGGCGCGAGTCATCGTTGAACCATGTCTT
GGCTTCCTGTAGAATCTGATTTAGGTNTNTNTTCTCTTTNNCNNTTTGGGGTTCCTGTTT
TCTGGATATNTNANGAGATGATGACAGTAACGAGGTATTGATAGTGGAGCACTAAAAATG
TTTGGAAAATAGTCTTGGCTTCCTGTAGAATCTGATT

Last Updated on 06.12.2018 16:11