Sequences from confirmation amplicon


Confirmation sequences for line 040H12 and hit At5g07690 are shown.
Line ID 040H12
Locus At5g07690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2447559
Primers used for PCR IF64 - 8474, show primer details
   
Sequence Name 47-K101384-0022-040-H12C-8409
GenBank Accession LN506149 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K101384-0022-040-H12C-8409
TCTACTTTGCCCAAAGAACAGATAACGAGATTAAGAACTACTGGAACACGCATCTCAAAA
AGCTCCTGATCGATAAGGGAATCGATCCCGTGACCCACAAGCCACTTGCCTATGACTCAA
ACCCGGATGAGCAATCGCAATCGGGTTCCATCTCTCCAAAGTCTCTTCCTCCTTCAAGCT
CCAAAAATGTACCGGAGATAACCAGCAGTGACGAGACACCGAAATATGATGCTTCCTTGA
GCTCCAAGAAACGTTGTTTTAAGAGATCGAGTTCTACATCAAAACTGTTAAACAAAGTTG
CAGCTAGGGCTTCTTCCATGGGAACTATACTAGGCGCCTCCATCGAAGGAACCTTGATCA
GCTCTACACCGTTGTCTTCATGTCTAAATGATGACTTTTCTGAAACAAGTCAATTTCAGA
TGGAAGAATTTGATCCATTCTATCAGTCATCTGAACACATAATTGATCATATGANAGAAG
ATATCAGCATCAACAATTCCGAATACGATNTCTCGCAGTTTCTCGAGCAGTTTAGTAACA
ACGAAGGGGGAAGAAGCTGACNATACTGGAGGAGGATATNACCNAGATCTTCTTATGTCT
GATGTCTCATCAACAAGCGTTGATGAAGACGAGAT
   
Sequence Name 47-K101394-0022-040-H12C-IF64
GenBank Accession LN506150 [GenBank]
Sequencing primer gene specific primer, IF64: 5'-TATGTTTTGCATCATCTCGTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K101394-0022-040-H12C-IF64
GAGATCTTGGTTATATCCTCCTCCAGTATTGTCAGCTTCCTCTCCTTCGTTGTTACTAAA
CTGCTCGAGAAACTGCGAGAAATCGTATTCGGAATTGTTGATGCTGATATCTTCTTTCAT
ATGATCAATTATGTGTTCAGATGACTGATAGAATGGATCAAATTCTTCCATCTGAAATTG
ACTTGTTTCAGAAAAGTCATCATTTAGACATGAAGACAACGGTGTAGAGCTGATCAAGGT
TCCTTCGATGGAGGCGCCTAGTATAGTTCCCATGGAAGAAGCCCTAGCTGCAACTTTGTT
TAACAGTTTTGATGTAGAACTCGATCTCTTAAAACAACGTTTCTTGGAGCTCAAGGAAGC
ATCATATTTCGGTGTCTCGTCACTGCTGGTTATCTCCGGTACATTTTTGGAGCTTGAAGG
AGGAAGAGACTTTGGAGAGATGGAACCCGATTGCGATTGCTCATCCGGGTTTGAGTCATA
GGCAAGTGGCTTGTGGGTCACGGGATCGATTCCCTTATCGATCAGGAGCTTTTTGAGATG
CGTGTTCCAGTAGTTCTTAATCTCGTTATCTGTTCTTTTGGGCAAATGTAGATGGCTTCA
TGTCCGGNAAATCTACATGGATCAGNCATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTTCATTTCAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11