Sequences from confirmation amplicon


Confirmation sequences for line 041B01 and hit At5g10860 are shown.
Line ID 041B01
Locus At5g10860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3429573
Primers used for PCR PA16 - 8474, show primer details
   
Sequence Name 75-K037021-022-041-B01C-8409
GenBank Accession LN513189 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K037021-022-041-B01C-8409
CATTTACAAAATGTCTCCAACTTTTGTTGATTTGGATGATCTCCCCCTTACAATGATCTT
CCGTAGATAATCTAGAGAAATCACAACGTCGAAAAGTGAATCACACATACCACAAGACAC
AACAAAAAAATTATTAATGATTAGATCTAATTTTACCTCTCTCTGTAATGATACCAGCAA
GAGCTTGTTGCTCACCAGGTTTCACAACCACCAAGGCACCAACATTGTGTTGTGTCATCT
AAACAAAATCAAACACAATCAATTCCAAATCCACATCGAGATATCTAAAAAAGGAAAAAG
AGAGTAGTATAAGCATACGGATTTAACAGCATCATAAACAGTGTCATCAGTAGTACACCA
AAGCCAAGATCCATCAGCACTTTTGCCTTTGGATTTCATGACATCGGAAATAGTTGTGCT
CTCAAATCCAG
   
Sequence Name 76-K037021-022-041-B01C-PA16
GenBank Accession LN513343 [GenBank]
Sequencing primer gene specific primer, PA16: 5'-ATCTGGATTCGAGAGCACAACTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K037021-022-041-B01C-PA16
GCAAAGTGCTGATGGATCTTGGCTTTGGTGTACTACTGATGACACTGTTCATGATGCTGT
TAAATCCGTATGCTTATACTACTCTCTTTTTCCTTTTTTAGATATCTCGATGTGGATTTG
GAATTGATTGTGTTTGATTTTGTTTAGATGACACAACACAATGTTGGTGCCTTGGTGGTT
GTGAAACCTGGTGAGCAACAAGCTCTTGCTGGTATCATTACAGAGAGAGGTAAAATTAGA
TCTAATCATTAATAATTTTTTTGTTGTGTCTTGTGGTATGTGTGATTCACTTTTCGGCAT
TGTGATTTCTCTAGATTATCTACGGAAGATCATTGTGCAAGGGAGATCATCCAAATCAAC
AAAAGTTGGAGACATTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCACTC

Last Updated on 06.12.2018 16:11