Sequences from confirmation amplicon


Confirmation sequences for line 041D08 and hit At4g15230 are shown.
Line ID 041D08
Locus At4g15230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8684204
Primers used for PCR EM01 - 8474, show primer details
   
Sequence Name 10-K023836-022-041-D08C-8409
GenBank Accession LN490476 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K023836-022-041-D08C-8409
GATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATACTCACATGTAAAC
TTGTGCTTTGTTTGAGAATTTAGTATCTCAAAACATTCACACTGATCTTAAACAATGAGT
TTACCTGAGGAGTTTCAATATAATACTGCACGTTTTGAAATGTGACAGTGAGTGGCTTAA
AAGGCAAGATAATTTTTCCTATGAAAAAGAGACGAAAGATGTGAAAAGATATCTTGTCAT
TACGCAAGCCACTGACCTAGTAAAACATAGAAAAGATGGAATAGCTGACAAAAAGAGATA
CCTGTTTTAGCTCGAGAAGTGATTTTAGGGCAGGGTTTAAAATCTTCTTCTATGGGCCGA
GAGTACTTTTCATGTGAGATGATAGCACGGGATCTTTGGGGATCTTAACTTG
   
Sequence Name 14-K023836-022-041-D08C-EM01
GenBank Accession LN492329 [GenBank]
Sequencing primer gene specific primer, EM01: 5'-TTATCTTTGCAAGTTAAGATCCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K023836-022-041-D08C-EM01
CCGTGCTATCATCTCACATGAAAAGTACTCTCGGCCCATAGAAGAAGATTTTAAACCCTG
CCCTAAAATCACTTCTCGAGCTAAAACAGGTATCTCTTTTTGTCAGCTATTCCATCTTTT
CTATGTTTTACTAGGTCAGTGGCTTGCGTAATGACAAGATATCTTTTCACATCTTTCGTC
TCTTTTTCATAGGAAAAATTATCTTGCCTTTTAAGCCACTCACTGTCACATTTCAAAACG
TGCAGTATTATATTGAAACTCCTCAGGTAAACTCATTGTTTAAGATCAGTGTGAATGTTT
TGAGATACTAAATTCTCAAACAAAGCACAAGTTTACATGTGAGTATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11