Sequences from confirmation amplicon


Confirmation sequences for line 041E09 and hit At5g55350 are shown.
Line ID 041E09
Locus At5g55350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22442379
Primers used for PCR 26nh - 8474, show primer details
   
Sequence Name 05-K114392-0022-041-E09C-8409
GenBank Accession LN488448 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114392-0022-041-E09C-8409
ATTTCATTGAATATATCCTATAATCATATGGATCATTTACAAAGGTAGAAAAACTTGTGC
TTGACGAAACCAATAAACAAAAAGGCTTCGTCAGCTAGTCTCTCGATCATGCCACTCCTT
GCAAGGTGAGGGAAAAACAACCAACCACCGGTCATAACAACAAATCCCACCGTGAGCAGT
CGTGAAACCATCTGACTCACCTTCCATCGCCGCACCAACCTCGTCCTCTTCGCAGCGCAT
TCAGCGGCAGTGCAAACTCCATGTAATACAAAGAACAAAGTGACTTCACCTGTCGGCGAT
TCACGTGTAAAATAGAAGAAAATAAGCTGNTGAACCA
   
Sequence Name 29-K114393-0022-041-E09C-26nh
GenBank Accession LN499395 [GenBank]
Sequencing primer gene specific primer, 26nh: 5'-GTGGTTCACGAGCTTATTTTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114393-0022-041-E09C-26nh
TTTGTTCTTTGTATTACTGGAGTTTGCACTGCCGCTGAATGCGCTGCGAAGAGGACGAGG
TTGGTGCGGCGATGGAAGGTGAGTCAGATGGTTTCACGACTGCTCACGGTGGGATTTGTT
GTTATGACCGGTGGTTGGTTGTTTTTCCCTCACCTTGCAAGGAGTGGCATGATCGAGAGA
CTAGCTGACGAAGCCTTTTTGTTTATTGGTTTCGTCAAGCACAAGTTTTTCTACCTTTGT
AAATGATCCATATGATTATAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAATTATT

Last Updated on 06.12.2018 16:11