Sequences from confirmation amplicon


Confirmation sequences for line 041H03 and hit At1g29890 are shown.
Line ID 041H03
Locus At1g29890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10465932
Primers used for PCR MP53 - 8474, show primer details
   
Sequence Name 01-K025944-022-041-H03C-8409
GenBank Accession LN486426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K025944-022-041-H03C-8409
GTAGATTCTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACTGTAAATGGCTA
AACCAGACCTTCCACGTCTACACGAATGGCATTTCAGATCTGGACTTGATCGCTACATAT
GGATCATCGGCATGATATATGCATATTTTCATCCCACTGTAATTCCAGAACCATGGTGCT
TTCATTTTTTCCATATCTCCTCTCGAAAGACGCAACTAAATATATTGGGGATTTCAGGTA
GAAAGATGGATGGAGAAATTGGAGGAGTGTGATGCTAAGAGAAGGATGTCAATCAAGACA
AGCATAATAGGAATTTCTTCATTCGTGAGTTGTTAGCTTTTCTCGGCTTCTGGTTCATCA
TATATAGTCTTCTCCACAATTATTCTGATGTTTGTGTCGTGGTTTCCAAATGTTCAGGCT
GGTTACCTTT
   
Sequence Name 02-K025944-022-041-H03C-MP53
GenBank Accession LN486882 [GenBank]
Sequencing primer gene specific primer, MP53: 5'-TATTCATACCAAAGGTAACCAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K025944-022-041-H03C-41F3
AATTTGGAAACCACGACACAAACATCAGAATAATTGTGGAGAAGACTATATATGATGAAC
CAGAAGCCGAGAAAAGCTAACAACTCACGAATGAAGAAATTCCTATTATGCTTGTCTTGA
TTGACATCCTTCTCTTAGCATCACACTCCTCCAATTTCTCCATCCATCTTTCTACCTGAA
ATCCCCAATATATTTAGTTGCGTCTTTCGAGAGGAGATATGGAAAAAATGAAAGCACCAT
GGTTCTGGAATTACAGTGGGATGAAAATATGCATATATCATGCCGATGATCCATATGTAG
CGATCAAGTCCAGATCTGAAATGCCATTCGTGTAGACGTGGAAGGTCTGGTTTAGCCATT
TACAGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATT
Primers used for PCR 14wp - 8474, show primer details
   
Sequence Name 26-K118496-0022-041-H03C-14wp
GenBank Accession LN498136 [GenBank]
Sequencing primer gene specific primer, 14wp: 5'-GTCACCAATAACAGGAAAGTCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118496-0022-041-H03C-14wp
TGGAAGGGTGGATGCAGGTACTGTGTTCGAACTTTACTCAGTAGTTTTCATTTTCCTACC
ATTTTATCCCTTACTTCCTTGCATTCACGTCAGCTTCATAATAGTATCTTCATCTAAATC
TTTGTGTGTCTTTTCTTTTGCATTCACCTCGATTCTCTAACTGATTTCCATCCCTTTGGG
ACTTTGCAGGTTCTATTTCTGATGTATCATTATTTTGCGGCAGTTGAGTTTTACAATGCA
ATCAGGGTCTTCATCGCTGGCTATGTGTGGATGACCGGTTTTGGGAACTTCTCTTATTAC
TATATCCGAAAGGATTTCTCCCTTGCACGATTCACTCAGGTCAGATCAACTATTTTCGAT
CACCATAGTTTGTTCAGTTTACCATGTGATGTTCTTTTAGAGAGCACTATGTCTTTCAAA
GCTCAAGATTTTTATGAAAGCTTTTACTTAATCCAGATGATGTGGCGGCTTAACTTTTTT
GTGGCGTTTTGTTGCATTATTCTCAACAATGACTATATGCTGTACTACATCTGTCCAATG
CACACTCTATTCACGCTGATGGTCTATGGAGCCCTTGGTATTTACAGTCAGTATAACGAA
ATAGCATCAGTGATGGCTCTGAAGATTGCTTCATGCTTTCTCGTGGTTATCCTTATGTGG
GAGATTCCTGGAGTTTTTGAGATTTTCTGGAGTCCTCTGGCATTCTTACTGGGTCAGTAA
CAAATTTCTCTCATTTTTCATACCATTTTTGTCATGTGGCTGATCTGTAGTTCTGTCTTC
TACACAAATATNTTGGATGGCATGCTTGAATAAAAATTGGTAACTAACGTTGATATTCTG
TCTTTAACACAAATACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAGAGTAATTA
   
Sequence Name 02-K118495-0022-041-H03C-8409
GenBank Accession LN487238 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118495-0022-041-H03C-8409
GCCATTTCATTGAATATATCCTGTATTTGTGTTAAAGACAGAATATCAACGTTAGTTACC
AATTTTTATTCAAGCATGCCATCCAAAATATTTGTGTAGAAGACAGAACTACAGATCAGC
CACATGACAAAAATGGTATGAAAAATGAGAGAAATTTGTTACTGACCCAGTAAGAATGCC
AGAGGACTCCAGAAAATCTCAAAAACTCCAGGAATCTCCCACATAAGGATAACCACGAGA
AAGCATGAAGCAATCTTCAGAGCCATCACTGATGCTATTTCGTTATACTGACTGTAAATA
CCAAGGGCTCCATAGACCATCAGCGTGAATAGAGTGTGCATTGGACAGATGTAGTACAGC
ATATAGTCATTGTTGAGAATAATGCAACAAAACGCCACAAAAAAGTTAAGCCGCCACATC
ATCTGGATTAAGTAAAAGCTTTCATAAAAATCTTGAGCTTTGAAAGACATAGTGCTCTCT
AAAAGAACATCACATGGTAAACTGAACAAACTATGGTGATCGAAAATAGTTGATCTGACC
TGAGTGAATCGTGCAAGGGAGAAATCCTTTCGGATATAGTAATAAGAGAAGTTCCCAAAA
CCGGTCATCCACACATAGCCAGCGATGAAGACCCTGATTGCATTGTAAAACTCAACTGCC
GCAAAATAATGATACATCAGAAATAGAACCTGCAAAGTCCCAAAGGGATGGAAATCAGTT
AGAGAATCGAGGTGAATGCAAAAGAAAAGACACACAAAGATTTAGATGAAGATACTATTA
TGAAGCTGACGTGAATGCAAGGAAGTAAGGGATAAAATGGTAGGAAAATGAAAACTACTG
AGTAAAGTTCGAACACAGTACCTGCATCCACCCTTTCCATTCTTCAGTCTGGTGA

Last Updated on 06.12.2018 16:11