Sequences from confirmation amplicon


Confirmation sequences for line 042B09 and hit At1g16830 are shown.
Line ID 042B09
Locus At1g16830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5761485
Primers used for PCR 16ud - 3144, show primer details
   
Sequence Name 35-K117029-0022-042-B09C-16ud
GenBank Accession LN501976 [GenBank]
Sequencing primer gene specific primer, 16ud: 5'-CAGTTTGAGAGCAAGTTTCCAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117029-0022-042-B09C-16ud
TATTGATACGAAAGTATACTGATCCGGCACATGTTTCCTTTTTCAAAACTAGTGAAAACC
TTACGAGCTTCTTCAAATCTTCCGAGTCTTGTGTACGTGTGTATCATCAAATTGCAAAGA
ACAATATCTGGAGCTAAACCTTCAGATTGCACTTTACTTAGCACGGTAAACGCTTCATCA
ACCATTCCTAAATCCACAAACCCTTTGATCAAACTAGTATACGTCACAAGATTAGGTGAG
CAACCAATCTGAATCATCTTATTGAACAGATCAACCGCCTTTTGCGGCTCTCCTGAACGA
AAAAACCCGCTAACCAACATGCTCCATACATTAACAGATACAGAGATTCCACTACAAATC
ATCAATCCAACTACTTGAAATATTCAATATTCAATTATTGGGAAACGACAATAATGGCTC
GATCTTTCTCTCGTTCACTTTCTCTATCCAAAGGCCGTTTCGCCGCCGCGTCATCGTGGT
TTCCCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAAT
GAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTT
ACGTCAGTGGAGATATCACTCCAATCCAC
   
Sequence Name 03-K117028-0022-042-B09C-3144
GenBank Accession LN487652 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117028-0022-042-B09C-3144
GTTTCATTTGGAGAGGACAGGTGTCCCGGGAGATCAGATTGTCGTTTCCCGCCTTCGGGA
AACCACGATGACGCGGCGGCGAAACGGCCGTTGGATAGAGAAAGTGAACGAGAGAAAGAT
CGAGCCATTATTGTCGTTTCCCAATAATTGAATATTGAATATTTCAAGTAGTTGGATTGA
TGATTTGTAGTGGAATCTCTGTATCTGTTAATGTATGGAGCATGTTGGTTAGCGGGTTTT
TTCGTTCAGGAGAGCCGCAAAAGGCGGTTGATCTGTTCAATAAGATGATTCAGATTGGTT
GCTCACCTAATCTTGTGACGTATACTAGTTTGATCAAAGGGTTTGTGGATTTAGGAATGG
TTGATGAAGCGTTTACCGTGCTAAGTAAAGTGCAATCTGAAGGTTTAGCTCCAGATATTG
TTCTTTGCAATTTGATGATACACACGTACACAAGACTCGGAAGATTCGAAGAAGCTCGTA
AGGTTTTCACTAGTTTGGAAAAAAGGAAACTTGTGCCGGATCAGTATACTTTTGCATCAA
TACTGTCTAGTCTATGTTTGTCTGGGAAGTTTGATCTCGTTCCTAGGATCACCCACGGTA
TAGGAACAGATTTTGATCTGGTTACTGGAAACT

Last Updated on 06.12.2018 16:11