Sequences from confirmation amplicon


Confirmation sequences for line 042B09 and hit At1g16840 are shown.
Line ID 042B09
Locus At1g16840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5763314
Primers used for PCR 07ft - 3144, show primer details
   
Sequence Name 87-K118629-0022-042-B09C-07ft
GenBank Accession LN514807 [GenBank]
Sequencing primer gene specific primer, 07ft: 5'-ATTTCACGTCTTTTCACTTGCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K118629-0022-042-B09C-07ft
GAGATCCTGAAGAGCCTGCAAGCTTCAATCTTTCTTTTATCAGAAACCCAACAAAATCAT
AAGACCTTGAGGATTCATGGATATAGATAGAAATGTGATTTGCTCAATCCATCAAGGTCA
TAACTTTAGTTTTGCCAAATAACTCACCCAAACAAGCAGTGTCATTTTTACACTTCCTCG
TCCTCAGAGTGAGACTCGGAATTTGAAGTTGTCTCGGTCTTGGTCTCCATCAATTGAGGT
TGAACACCTAAACCCAATAAACAAAAACCATATGTCAACGAATGACCAACAATCAAACAA
TTCTCATACACCACAACTAGTTAAGAACCTGTTCCACCAAAGACGCTAGAAAAATTCTCA
TACTCATATGTGATGTTGCACGGTAAACGCTTCATCAACCATTCCTAAATCCACAAACCC
TTTGATCAAACTAGTATACGTCACAAGATTAGGTGAGCAACCAATCTGAATCATCTTATT
GAACAGATCAACCGCCTTTTGCGGCTCTCCTGAACGAAAAAACCCGCTAACCAACATGCT
CCATACATTAACAGATACAGAGATTCCACTACAAATCATCAATCCAACTACTTGAAATAT
TCAATATTCAATTATTGGGAAACGACAATAATGGCTCGATCTTTCTCTCGTTCACTTTCT
CTATCCAAAGGCCGTTTCGCCGCCGCGTCATCGTGGTTTCCCGAAGGCGGGAAACGACAA
TCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGG
GTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
   
Sequence Name 68-K118628-0022-042-B09C-3144
GenBank Accession LN512102 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K118628-0022-042-B09C-3144
CCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGGAAACCACGATGACGCGGCGGCGAAACGGC
CTTTGGATAGAGAAAGTGAACGAGAGAAAGATCGAGCCATTATTGTCGTTTCCCAATAAT
TGAATATTGAATATTTCAAGTAGTTGGATTGATGATTTGTAGTGGAATCTCTGTATCTGT
TAATGTATGGAGCATGTTGGTTAGCGGGTTTTTTCGTTCAGGAGAGCCGCAAAAGGCGGT
TGATCTGTTCAATAAGATGATTCAGATTGGTTGCTCACCTAATCTTGTGACGTATACTAG
TTTGATCAAAGGGTTTGTGGATTTAGGAATGGTTGATGAAGCGTTTACCGTGCAACATCA
CATATGAGTATGAGAATTTTTCTAGCGTCTTTGGTGGAACAGGTTCTTAACTAGTTGTGG
TGTATGAGAATTGTTTGATTGTTGGTCATTCGTTGACATATGGTTTTTGTTTATTGGGTT
TAGGTGTTCAACCTCAATTGATGGAGACCAAGACCGAGACAACTTCAAATTCCGAGTCTC
ACTCTGAGGACGAGGAAGTGTAAAAATGACACTGCTTGTTTGGGTGAGTTATTTGGCAAA
ACTAAAGTTATGACCTTGATGGATTGAGCAAATCACATTTCTATCTATATCCATGAATCC
TCAAGGTCTTATGATTTTGTTGGGTTTCTGATAAAAGAAAGATTGAAGCTTGCAGGCTCT
TCAGGATCTCTTGTCAAGACCAAGAGCTGCCGAATTCGGACAACCAAGAGCAAGTGAAA
Primers used for PCR 76ww - 8474, show primer details
   
Sequence Name 62-K118893-0022-042-B09C-76ww
GenBank Accession LN510865 [GenBank]
Sequencing primer gene specific primer, 76ww: 5'-CAAAACTCAAAATTACAGCGTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118893-0022-042-B09C-76ww
TTTCTTCGGATCCTTCTAGAAATGGCTCGATCTTTCTCTCGTTCACTTTCTCTATCCAAG
GCCGTTTCGCCGCCGCGTCATCCTCTCTCCTCCCTTCGTCGCACCTTGTTTCATTCCGAT
CTCAATCTTCCGATCGTCGGGGAGATCTGTACGAGATTGATACCTCCGCGGCTTCTCAAT
CGCCGTCGGATCCTTTAATACAGAAGCTAGAAGACGCCGTTCACCGGATTTTTGTGCGCC
GAGCTCAGCCTGATTGGCTCCCTTTTGTTCCTGGTGCGTCTTATTGGGTCCCTCCTCCTG
GATCTGGATCTCAGTCTCATGGAATCGCTCAGCTCGTTGTGAAACTCGCGAATCCGTTAA
CACACGAAGAATCTTTATCTACCAATTCTTCTCATGGATGGCCTTCCTCCGATTATTTCC
TTAAAGGTTCTTATTGGTCATTCTCTCTCTGGTTTTTTATGGATAAAGCTTGGATTGGAA
TCTTCAATATGTTACTCATGCTTTAGCTACCAACTAAATTGATTTTGAAATTGATCAAAT
TTTTGTTGAATGTAAGCATTCATATGATTTAACTAGTGGCAGGTTTTTGATATGTTGATA
ATGCTATGTCAATCGTAAAGTTAGTCATTGCTCGATGCTGTTACAGGTTCTGAATGGGAG
ATCATTAATATGTGATTAACATTTTTGAGAAATAGAACGAGAAAAAGATTAGGCTAAACC
ACAATTGAATGTGATTAATATTCAATTGTAAATGGCTTCATGTCCGGGGAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAATG
   
Sequence Name 22-K118892-0022-042-B09C-8409
GenBank Accession LN496331 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118892-0022-042-B09C-8409
CATTTCATTGAATATTAATCACATTCAATTGTGGTTTAGCCTAATCTTTTTCTCGTTCTA
TTTCTCAAAAATGTTAATCACATATTAATGATCTCCCATTCAGAACCTGTAACAGCATCG
AGCAATGACTAACTTTACGATTGACATAGCATTATCAACATATCAAAAACCTGCCACTAG
TTAAATCATATGAATGCTTACATTCAACAAAAATTTGATCAATTTCAAAATCAATTTAGT
TGGTAGCTAAAGCATGAGTAACATATTGAAGATTCCAATCCAAGCTTTATCCATAAAAAA
CCAGAGAGAGAATGACCAATAAGAACCTTTAAGGAAATAATCGGAGGAAGGCCATCCATG
AGAAGAATTGGTAGATAAAGATTCTTCGTGTGTTAACGGATTCGCGAGTTTCACAACGAG
CTGAGCGATTCCATGAGACTGAGATCCAGATCCAGGAGGAGGGACCCAATAAGACGCACC
AGGAACAAAAGGGAGCCAATCAGGCTGAGCTCGGCGCACAAAAATCCGGTGAACGGCGTC
TTCTAGCTTCTGTATTAAAGGATCCGACGGCGATTGAGAAGCCGCGGAGGTATCAATCTC
GTACAGATCTCCCCGACGATCGGAAGATTGAGATCGGAATGAAACAAGGTGCGACGAAGG
GAGGAGAGAGGATGACGCGGCGGCGAAACGGCCTTTGGATAGAGAAAGTGAACGAGAGAA
AGATCGAGCCATTATCTAGAAGGATCCGAAGAAAAGAGAATCAGCGACGAGATCGGTTTC
TGTTTCAACGCTGTATTTTTTGAGTTT

Last Updated on 06.12.2018 16:11