Sequences from confirmation amplicon


Confirmation sequences for line 042D11 and hit At1g07360 are shown.
Line ID 042D11
Locus At1g07360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2262380
Primers used for PCR 14so - 3144, show primer details
   
Sequence Name 41-K116549-0022-042-D11C-14so
GenBank Accession LN504221 [GenBank]
Sequencing primer gene specific primer, 14so: 5'-ATACTGAGAGATCATGAAGCCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116549-0022-042-D11C-14so
GCGAACTTGTCTCGGTGACACCCCTACGTGCGAATGGTGATTTCTCGATTCCTTCTCTCA
AACATTCTTGGATTTTGCTATTGCTTGTTTTGTTTCAATGGCTCCTGAATCTTATTTCCG
GATCTCATCTCTTAACCAGTCTCTTGCTCGTTGATGTTTTCTGTATATTGGAGTGGTTAA
CTTAGTAAATATGGTTTTAGGGTTTAGCTTGTTATCAAATTCATCGAAAAATTGGGTTTT
ACATCCAAATTCGTTTAGTTATAGTTGGATTTGATTACAGCTGCGGATTCTCTTAGCTTT
CAGCTGCAATTTTGTTTTCTGAAAATCTCGATGTTTTGATTTCTGATCAAACACTGATAG
TTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGA
AATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCC
CTTACGTCAGTGGAGATAT
   
Sequence Name 01-K116548-0022-042-D11C-3144
GenBank Accession LN486735 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116548-0022-042-D11C-3144
CTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATCAGAAATCAA
AACATCGAGATTTTCAGAAAACAAAATTGCAGCTGAAAGCTAAGAGAATCCGCAGCTGTA
ATCAAATCCAACTATAACTAAACGAATTTGGATGTAAAACCCAATTTTTCGATGAATTTG
ATAACAAGCTAAACCCTAAAACCATATTTACTAAGTTAACCACTCCAATATACAGAAAAC
ATCAACGAGCAAGAGACTGGTTAAGAGATGAGATCCGGAAATAAGATTCAGGAGCCATTG
AAACAAAACAAGCAATAGCAAAATCCAAGAATGTTTGAGAGAAGGAATCGAGAAATCACC
ATTCGCACGTAGGGGTTGTCACCGAGACAAGATTCGCAGATGATTGGGAAATCGGAGCGT
TCCCATCCATCGGCTT

Last Updated on 06.12.2018 16:11