Genotyping and primer details


Genotyping and primer details for 042D11 - At1g07360
Line ID 042D11
Locus At1g07360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2262380 of line 042D11 Chr1:2262380
   
Insertion allele amplicon 3144 - 14so
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 14so: 5'-ATACTGAGAGATCATGAAGCCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2262783
   
wt amplicon (over InsPos) 19ti - 14so
wt amplicon start Chr1:2261902
wt amplicon end Chr1:2262783
wt amplicon size 882 bp
Locus specific primer 14so: 5'-ATACTGAGAGATCATGAAGCCGAT-3'
2nd locus specific primer 19ti: 5'-AAAATAGTATCATTGGGTCGCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11