Sequences from confirmation amplicon


Confirmation sequences for line 042F09 and hit At3g15470 are shown.
Line ID 042F09
Locus At3g15470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5217056
Primers used for PCR CW11 - 8474, show primer details
   
Sequence Name 43-K104125-0022-042-F09C-CW11
GenBank Accession LN504818 [GenBank]
Sequencing primer gene specific primer, CW11: 5'-CTTGATGGCTCTAAAAGTTCCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K104125-0022-042-F09C-CW11
TTTGTTCCTGGGAGCTCATCTGAAGTGCTTGTCACATCTTCCGATTCAAGGATCCGTGTT
GTTGATGGAACTGATCTAGTTAACAAACTTAAAGGTAAACAGACAAACAATCGCCTTTTG
AAGTTTCAAGCTTTGTCTTCTGTTTACGAGCCAAAGTATCGATCTTTTCTGTTTTCATTT
CATTTGATAGGGTTTCGAAATACGAGCAGCCAGATCTCTGCCTCAATCACAGCAGATGGG
AAATACGTAGTTTCAGCAAGCGAGGATTCACACGTGTACATATGGAAGTACGAATCCCCT
GCTTCTCGACCAAGCAGAAGTAACAACAACAAGAACGTGACAGTCACAAACTCTTACGAG
CATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATATA
GATGTCCGCA
   
Sequence Name 59-K104126-0022-042-F09C-8409
GenBank Accession LN509986 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K104126-0022-042-F09C-8409
TTACAATTGAATATGCTCGTAAGAGTTTGTGACTGTCACGTTCTTGTTGTTGTTACTTCT
GCTTGGTCGAGAAGCAGGGGATTCGTACTTCCATATGTACACGTGTGAATCCTCGCTTGC
TGAAACTACGTATTTCCCATCTGCTGTGATTGAGGCAGAGATCTGGCTGCTCGTATTTCG
AAACCCTATCAAATGAAATGAAAACAGAAAAGATCGATACTTTGGCTCGTAAACAGAAGA
CAAAGCTTGAAACTTCAAAAGGCGATTGTTTGTCTGTTTACCTTTAAGTTTGTTAACTAG
ATCAGTTCCATCAACAACACGGATCCTTGAATCGGAAGATGTGACAAGCACTTCAGATGA
GCTCCCAGGAACAAACTAAAATATGAAATGAGACAAAGCAAGGAACTTTTAAGCCCATCA
A

Last Updated on 06.12.2018 16:11