Sequences from confirmation amplicon


Confirmation sequences for line 042G07 and hit At5g53300 are shown.
Line ID 042G07
Locus At5g53300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21633714
Primers used for PCR AU01 - 8474, show primer details
   
Sequence Name 07-K106284-0022-042-G07C-8409
GenBank Accession LN489281 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106284-0022-042-G07C-8409
GTTCTGATTTCTCTGGTGAGATCTTATAAAGGCTTAAAACTCGTTTCATTGTCTTAGTTT
CTGGGGTCTGGTTATACTTGTGAAGAAAAAACTGTCTCTTTGTTTCCTTGTGCTTATGTG
GTGTGAGATTGAGTTTATCTTTCTGTGTACAGGTCCTGTTGCTGAAGACATGTTTCACTG
GCAGGCAACGATAATGGGTCCTTCAGAGAGTCCTTATGCTGGAGGTGTTTTCCTTGTAAC
CATCCATTTCCCTCCGGATTACCCATTCAAGCCTCCTAAGGTGATTCCAATATTTTGAGA
ATTGTTATCAAGTATGTTTTGCCAAAGCTCGTATGTTCTTTATGGTTGCTTCATTATCTC
ATTGTTCTTCATTATGGTGTGTGTGATGATATCTAGGTGGCCTTTAGGACCAAGGTGTTC
CATCCCAACATTAACAGCAACGGTAGCATTTGCCTCGACATCTTGAAGGAGCAGTGGAGT
CCTGCGCTCACCATTTCCAAGGTTCGGTTTCTCTTATTCTTTTGTTAACAATAGAAACCC
ACCTTAAAACAAATACTCTCTATTAAAACTGCTTCAGAGGCAGTAATCTACTCTTATCAA
ACATGCTTTTGCTCCTTAACCCCTAG
   
Sequence Name 07-K106285-0022-042-G07C-AU01
GenBank Accession LN489282 [GenBank]
Sequencing primer gene specific primer, AU01: 5'-AACTAGGGATTAAGGAGCAAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106285-0022-042-G07C-AU01
GCCTCTGAGCAGTTTTAATAGAGAGTATTTGTTTTAAGGTGGGTTTCTATTGTTAACAAA
AGAATAAGAGAAACCGAACCTTGGAAATGGTGAGCGCAGGACTCCACTGCTCCTTCAAGA
TGTCGAGGCAAATGCTACCGTTGCTGTTAATGTTGGGATGGAACACCTTGGTCCTAAAGG
CCACCTAGATATCATCACACACACCATAATGAAGAACAATGAGATAATGAAGCAACCATA
AAGAACATACGAGCTTTGGCAAAACATACTTGATAACAATTCTCAAAATATTGGAATCAC
CTTAGGAGGCTTGAATGGGTAATCCGGAGGGAAATGGATGGTTACAAGGAAAACACCTCC
AGCATAAGGACTCTCTGAAGGACCCATTATCGTTGCCTGCCAGTGAAACATGTCTTCAGC
AACAGGACCTGTACACAGAAAGATAAACTCAATCTCACACCACATAAGCACAAGGAAACA
AAGAGACAGTTTTTTCTTCACAAGTATAACCAGACCCCAGAAACTAAGACAATGAAACGA
GTTTTAAGCCTTTATAAGATCTTCACCAGAGAAATCAGAAACGAGATAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCC

Last Updated on 06.12.2018 16:11