Sequences from confirmation amplicon


Confirmation sequences for line 043E04 and hit At5g51640 are shown.
Line ID 043E04
Locus At5g51640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20976964
Primers used for PCR 01fx - 8474, show primer details
   
Sequence Name 29-K111953-0022-043-E04C-01fx
GenBank Accession LN499352 [GenBank]
Sequencing primer gene specific primer, 01fx: 5'-GGACTCCATCTGATTACGAGCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K111953-0022-043-E04C-01fx
CTCAGAAATTTCTTAGCATCAAAACGCGGAAGATCACACTGAGACGGTTTCCATCTCCAG
TTTTCATACCCCTTGTCAGGTCTTCCATTGCCCTGACAGTTTTGCATCTGTGTCAAAAGA
GGACAAGAGTTGTTTGTATACAACGGTCCCATCGGATCATAAAACCAGTTACCATGATAT
AAATCACATTCTGCAAAATGGTCAAAGCAAATCAGAAAAGCTTCAGTAAGAAAAGTGATA
AACACAATTTCAAAAAAATCCTGAGAGAAGACATGAAGGAGACATAATTGTCAAAAGGTA
GTTATATTTTACCAAATCAGATAACTATTAGTATAAAGGAGGTACCTAACTATATAGTCC
AAAGGTCATTAAGATTCCAGATTAAGGACTTAAGAGTTAAATGCATCATTAATTAGTAGC
ATTGGTTTTTTGTCTCAGTGATCCTTCAAGAGTAATTAAGTCAAATCATCATTAAATAGC
TACTTAACTTACTCCACATAAATTTGCACACTAATCTACAACAATTAGAGAAACTTAAGC
AATCACTTAACTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 13-K111952-0022-043-E04C-8409
GenBank Accession LN492101 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K111952-0022-043-E04C-8409
TCCTGAGTTAAGTGATTGCTTAAGTTTCTCTAATTGTTGTAGATTAGTGTGCAAATTTAT
GTGGAGTAAGTTAAGTAGCTATTTAATGATGATTTGACTTAATTACTCTTGAAGGATCAC
TGAGACAAAAAACCAATGCTACTAATTAATGATGCATTTAACTCTTAAGTCCTTAATCTG
GAATCTTAATGACCTTTGGACTATATAGTTAGGTACCTCCTTTATACTAATAGTTATCTG
ATTTGGTAAAATATAACTACCTTTTGACAATTATGTCTCCTTCATGTCTTCTCTCAGGAT
TTTTTTGAAATTGTGTTTATCACTTTTCTTACTGAAGCTTTTCTGATTTGCTTTGACCAT
TTTGCAGAATGTGATTTATATCATGGTAACTGGTTTTATGATCCGATGGGACCGTTGTAT
ACAAACAACTCTTGTCCTCTTTTGACACAGATGCAAAACTGTCAGGGCAATGGAAGACCT
GACAAGGGGTATGAAAACTGGAGATGGAAACCGTCTCAGTGTGATCTTCCGCGTTTTGAT
GCTAAGAAATTTCTTGAGCTAATGAGAGGCAAGACACTAGCTTTTATAGGCGATTCAGTA
GCTCGTAATCAATTTGGAGT

Last Updated on 06.12.2018 16:11