Sequences from confirmation amplicon


Confirmation sequences for line 044E09 and hit At1g12560 are shown.
Line ID 044E09
Locus At1g12560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4276806
Primers used for PCR 01ok - 8474, show primer details
   
Sequence Name 34-K114805-0022-044-E09C-01ok
GenBank Accession LN501520 [GenBank]
Sequencing primer gene specific primer, 01ok: 5'-ATCATTGAACAATGTCGTGCTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K114805-0022-044-E09C-01ok
GGTTTCCGTACCCCATGCACCACCTACATTATATATATATATATATACATAAAGTTATTA
TATCATTGATCTTATGCGTGACATATTGTGATTCAGGTACGGTTCAATGCACATGCAATG
CAAATGAAAAATTGCTATGGACTACGTATCCCTGGTAAAATATTTTGGAAACTTTTTTCA
TAAACTTTTTTTTTTTTTAAACCGGGGGAATATTTTTTTCGGAAACCTTTGAACCCCCCC
CTTCATATGGTATATCCAAAAAATCCATTTGTTATTTCCGGAAAATTTCCATTTGAAAAG
GGCTCCTTTCCCGGAAATTCTCCTGGAATAACCATTAATTATAAGGTTCAATATGAAAAA
AAAAAAAAATTATTTCCCATTTTTTTTCCATTTCAAAAATGTAAATTTCCCCCCTTTTTA
AAAAA
   
Sequence Name 10-K114804-0022-044-E09C-oriL
GenBank Accession LN490774 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114804-0022-044-E09C-oriL
CATATACCATATGAAGTCGAAGATCCAACGTTTCCAGAACATATATATCCACACGATTAA
CAAAAAAAAAAGTTTTTGAAAAAAGTTTTCCAAAATTTTTACCCTGGGATCCTTATTCCA
AACCATTTTTTCTTTTGCTTGGCAGGTGCTTGGACCCGTACCTGATTCACAATTTGTCCC
GCAAAAAACCATGGATTTAAAACCTTTTGGTATTTTTTTTTTTTTTTAAGGTGGGGGGGG
CAGGGGGGTACGAAACCTTTTTTAACAGGGGCAACGGATTGTCCCGGGGGGCCTTAACCA
AAATT

Last Updated on 06.12.2018 16:11