Sequences from confirmation amplicon


Confirmation sequences for line 044F02 and hit At4g13990 are shown.
Line ID 044F02
Locus At4g13990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8085421
Primers used for PCR 05wx - 8474, show primer details
   
Sequence Name 58-K118874-0022-044-F02C-05wx
GenBank Accession LN509841 [GenBank]
Sequencing primer gene specific primer, 05wx: 5'-GTAGGTATCTTTGGGGTTTCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K118874-0022-044-F02C-05wx
AGGACGGCTTGTGGTACAGAAAGAGTGGGGTCGAATGTCTGGTAGAGACCATTTCTTGGT
TTCAGGAAGGATTGCTTGGGATTTCAGGAGACAGACTGATAACGAATCTGATTGGGGAAG
CAAGCTTCGGTTCTTACCCGAGTCGCGGAACATGTCGATGTTGTCCATCGAATCGAGTTC
TTGGAAGAATGATTACGCTATTCCGTATCCGACTTGTTTCCATCCAAGATCAGTGGATGA
GATTGTGGAGTGGCAGGAGCTAATGAGGTCTCGAAAACGCGAGTATCTATTCACCTTTGC
GGGTGCTCCAAGGCCTGAATATAAAGACTCGGTTCGAGGGAAGATTATAGATGAGTGTTT
GGAGTCAAAGAAGCAATGTTACTATGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 18-K118873-0022-044-F02C-8409
GenBank Accession LN494528 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118873-0022-044-F02C-8409
CCTTACATTGAATATATCCATAGTAACATTGCTTCTTTGACTCCAACACTCATCTATAAT
CTTCCCTCGAACCGAGTCTTTATATTCAGGCCTTGGAGCACCCGCAAAGGTGAATAGATA
CTCGCGTTTTCGAGACCTCATTAGCTCCTGCCACTCCACAATCTCATCCACTGATCTTGG
ATGGAAACAAGTCGGATACGGAATAGCGTAATCATTCTTCCAAGAACTCGATTCGATGGA
CAACATCGACATGTTCCGCGACTCGGGTAAGAACCGAAGCTTGCTTCCCCAATCAGATTC
GTTATCAGTCTGTCTCCTGAAATCCCAAGCAATCCTTCCTGAAACCAAGAAATGGTCTCT
ACCAGACATTCGACCCCACTCTTTCTGTACCACAAGCCAATCCATCAGCTCATGAGACGA
AGAATCTCTAACCGTAATGTTGAAACCCCAAGAATACCTA

Last Updated on 06.12.2018 16:11