Sequences from confirmation amplicon


Confirmation sequences for line 044G12 and hit At3g17480 are shown.
Line ID 044G12
Locus At3g17480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5983650
Primers used for PCR 03we - 8474, show primer details
   
Sequence Name 57-K118393-0022-044-G12C-03we
GenBank Accession LN509545 [GenBank]
Sequencing primer gene specific primer, 03we: 5'-TCAGTGATATCATTTGTCACCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118393-0022-044-G12C-03we
CAGACTGAAGCCGCTCTTCTCTTACAGAAGATAGTGAGATCACATCGCTATACCAACTAG
TTTGAAACGGAAGGCACACATGTGGCCCAAATCTTTCTCTTGTAAAATCAAAACTTGTTA
AGAAACAATTGACTAATTCTCCATCTACTTCATGATCATCTATAACGATCCAATAAGTAT
TCCCCTTTACTGACATGCCTTGGCTACCTATGTCTAGTTCGAAGTCTATTTCGACACTAT
CAAGAACCCTCCATGAACTCGAATTAAACTCGTAGATTTCCAACTCAAAATTAGTTCCAT
CATCCAAGAACCTTAGGATTTTGTAATCACGGCACAAATTTTTGTTTTCGTATCCGAGAG
TAATGATGGAGAAAAAGAAAGAGTAAATGGCTTTCATACTCATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 09-K118392-0022-044-G12C-8409
GenBank Accession LN490398 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118392-0022-044-G12C-8409
TTACATTGAAATGAGTATGAAAGCCATTTACTCTTTCTTTTTCTCCATCATTACTCTCGG
ATACGAAAACAAAAATTTGTGCCGTGATTACAAAATCCTAAGGTTCTTGGATGATGGAAC
TAATTTTGAGTTGGAAATCTACGAGTTTAATTCGAGTTCATGGAGGGTTCTTGATAGTGT
CGAAATAGACTTCGAACTAGACATAGGTAGCCAAGGCATGTCAGTAAAGGGGAATACTTA
TTGGATCGTTATAGATGATCATGAAGTAGATGGAGAATTAGTCAATTGTTTCTTAACAAG
TTTTGATTTTACAAGAGAAAGATTTGGGCCACATGTGTGCCTTCCGTTTCAAACTAGTTG
GTATAGCGATGTGATCTCACTATCTTCTGTAAGAGAAGAGCGGCTTTCAGTCTTGTTTCA
TGAAGAAGATTCTTTAACGATGGAGATATGGGTGAC

Last Updated on 06.12.2018 16:11