Sequences from confirmation amplicon


Confirmation sequences for line 045B12 and hit At5g03530 are shown.
Line ID 045B12
Locus At5g03530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:886736
Primers used for PCR 01cc - 8474, show primer details
   
Sequence Name 20-K108223-0022-045-B12C-01cc
GenBank Accession LN495243 [GenBank]
Sequencing primer gene specific primer, 01cc: 5'-AGAACAAGTAGTGCGATTTCGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K108223-0022-045-B12C-01cc
CCACGAGCATCCTTACACATTCTTGGTTTGTGGAGTAAAGCTCAATCTCCTTACCCCACA
CATCTACTAAGTTTGTAAATGTCTCCCTTCTCGTCACATCATAAACTGCATTCACCAAGA
AACATTAACTAAACAGTTTACAGACAGAGAGATCTCTTAGTCTCCTTTGATTGATTGATT
GATGATGGACGTTACCGAGAATGATTCCTTGGGCGCCTCTGTAGTAAGAGCTTGTCAGTG
TTCTAAACCGTTCCTGTCCAGCTACAATGAGAAAACCGAGAATATTATCAGAACCATTAG
TTTTATTTACAATAAAACTTCTATAATAATCTCAAAATATAGAATCATTCACCTGTGTCC
CAGATTGTGAGTTTTAGCCTTTTGCCTCCTACTGTCAATTGTTTGATCTTGAAATCAACA
CCTATGAGTAATACTTATGTTAAACCAACAATATAATGCCAATAAGATCCGACAAAACAG
ACTTCCTTAAACCAAACCAAGACCAGACTGCTAGATTATCAGACAGAAAGTATCTAACTG
GGTCTAACCAAAAAAGTCAATATTGTTTCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 04-K108222-0022-045-B12C-8409
GenBank Accession LN487950 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K108222-0022-045-B12C-8409
ATTTACATTGAATATATCCTGAAACAATATTGACTTTTTTGGTTAGACCCAGTTAGATAC
TTTCTGTCTGATAATCTAGCAGTCTGGTCTTGGTTTGGTTTAAGGAAGTCTGTTTTGTCG
GATCTTATTGGCATTATATTGTTGGTTTAACATAAGTATTACTCATAGGTGTTGATTTCA
AGATCAAACAATTGACAGTAGGAGGCAAAAGGCTAAAACTCACAATCTGGGACACAGGTG
AATGATTCTATATTTTGAGATTATTATAGAAGTTTTATTGTAAATAAAACTAATGGTTCT
GATAATATTCTCGGTTTTCTCATTGTAGCTGGACAGGAACGGTTTAGAACACTGACAAGC
TCTTACTACAGAGGCGCCCAAGGAATCATTCTCGGTAACGTCCATCATCAATCAATCAAT
CAAAGGAGACTAAGAGATCTCTCTGTCTGTAAACTGTTTAGTTAATGTTTCTTGGTGAAT
GCAGTTTATGATGTGACGAGAAGGGAGACATTTACAAACTTAGTAGATGTGTGGGGTAAG
GAGATTGAGCTTTACTCCACAAACCAAGAATGTGTAAGGATGCTCGTTGGGAACAAAGTC
GATAGAGTGAGTTGCAATCGAAATCGCACTCCTTGTTC

Last Updated on 06.12.2018 16:11