Sequences from confirmation amplicon


Confirmation sequences for line 045E01 and hit At5g04170 are shown.
Line ID 045E01
Locus At5g04170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1145618
Primers used for PCR 39fr - 8474, show primer details
   
Sequence Name 17-K111563-0022-045-E01C-39fr
GenBank Accession LN493907 [GenBank]
Sequencing primer gene specific primer, 39fr: 5'-AGCTCCTTATCATCAATAAAGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K111563-0022-045-E01C-39fr
GCAGCCACTATATTCGGATCTGTCCCCGGAGCAAAACCTGACGGAATCAGAGAAGCAAAA
GGACTTCCATAAGAGGCTTGCGGAGGATAACCTCCGTATCCGCCACCGTGACCAGAAGAC
GCTGGACGAGGTGGAGCACCACCGTAACTTCCATAGTCAGAAACCCCATGCGGTGGAGTA
GCTCCGTATCCTCCACCGTGCCCAGACGGCTGCGACGGACGAGGTCCAGCACCGTAACTT
CCGTAATCTGAAGATCCAGACGGTGGTGGAGCTCCGTAGCCTCCTCCGTATGGTTTCTCC
TTTGGCGGCTTGTTGTAGTCTCCTGGAGAAGGCGCGTACGGAGCTGAAGGAGGCGGTGCT
CCGTATGATGAAGATCCGTACGTCGGCGCAGAAGAGGATGATGATTGTGGTTTAGATGCG
CCGTAGGGTACAGCGTAAGGAGAAGAGGTTGTTGATGATCCGTAGGGAGGATTGTTTCCG
CCGGAAGAGTAAGGTGGTTGAGGTGGTGGAGGTTGATTACCGCCGCCNTAACCATGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATATAGATGTCCGCA
   
Sequence Name 01-K111556w-0022-045-E01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K111556w-0022-045-E01C-8409
GGCGGTAATCANNACCTCCACCACCTCAACCANNCCTTACTCTTCCGGGGCGGAAACANN
ATCCTCCCTACNNGGATCATCAACAANNCCTCTTCNNTCCTTACGCTGNNTACCCTACGG
GGCGCANNTCTAAACCANNCAATCATCANNTCCTCTTNNCTGCGCCGAACGTACGGGAAN
TCTTCATCNNATACGGAAAGCACCGCCTNNCCTTCAGCTNNCCGTACGCGNNCCTTCTCC
AGGGAGAAACTACAACNNAAGCCGCCAAAGGGAGAAAANNCCATACGGAGGGAGGGCTAN
NCGGAGCTCNNCACCACCGTCTGGGGATCTTCAGAATNNTACGGAAGTTACGNGGNTGCT
GGACCNTNNCGTCCGTCNNGCAGCCGTCTGGGGCNNACGGTGGAAGGATACGGNNNAGCT
ACTCCACNNCGCATGGGGGGTTTNNCTGACTATNNGGAAGTTACGGGGTGGTGNNCTCCA
CCTNNCGTCCAGCNNGTCTTCTGNNGTCACGGTNNGGCGGAAATACGGAGGGGTTAT

Last Updated on 06.12.2018 16:11