Sequences from confirmation amplicon


Confirmation sequences for line 046D04 and hit At1g14530 are shown.
Line ID 046D04
Locus At1g14530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4972726
Primers used for PCR MI21 - 8474, show primer details
   
Sequence Name 45-K022563-022-046-D04C-8409
GenBank Accession LN505387 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K022563-022-046-D04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATACGTAATTCAGGTGAAACTA
ACTACCTATTTGGCCTTTGTAATATCTGAGAAATTTTCAAAGTTTAGAGTTAACCTTGAA
CTTGGTGGACCTGTGGGCCTGAGCTTCTTATTGAATTTCCTTACGATCCATTTGTGTTGT
GGGTTCCTTAGCTTTAATCTTTCATTTGAATGTATTAATTTCAGATTGCTCTTTGGTTGG
TTTTGTGGTGGAAGCCTGTTCACCTTATGGTAATCATTTCTAAGATGTTCTTTGCAGGTA
TAGTTTGAAGTATAGATCATTTTACACAGAGATTGATTTTTTTAATGCACTCTATGTCTC
ATCTCTTGTGAATGTATATTGCAGGTGTCTCATTGTTTGCGGCCCTTGGCTTTTTATTAT
ATGGAGGAAGGTAAGTATGT
   
Sequence Name 46-K022563-022-046-D04C-MI21
GenBank Accession LN505734 [GenBank]
Sequencing primer gene specific primer, MI21: 5'-TGCAAGTGCAACATACTTACCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K022563-022-046-D04C-MI21
CATATAATAAAAAGCCAAGGGCCGCAAACAATGAGACACCTGCAATATACATTCACAAGA
GATGAGACATAGAGTGCATTAAAAAAATCAATCTCTGTGTAAAATGATCTATACTTCAAA
CTATACCTGCAAAGAACATCTTAGAAATGATTACCATAAGGTGAACAGGCTTCCACCACA
AAACCAACCAAAGAGCAATCTGAAATTAATACATTCAAATGAAAGATTAAAGCTAAGGAA
CCCACAACACAAATGGATCGTAAGGAAATTCAATAAGAAGCTCAGGCCCACAGGTCCACC
AAGTTCAAGGTTAACTCTAAACTTTGAAAATTTCTCAGATATTACAAAGGCCAAATAGGT
AGTTAGTTTCACCTGAATTACGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
Primers used for PCR FL93 - 8474, show primer details
   
Sequence Name 49-K029745-022-046-D04C-8409
GenBank Accession LN506724 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K029745-022-046-D04C-8409
TCTCCATATTGACCATCATACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGCCA
TTTACAATTGTGAAGAAACTTGGCCTCAGTCCATCAGTCGATACAGCACGTGCCTGCCAA
GATAGAGTAAATATCAACAACAATGCAGGAAAAACTAAATTGAAAATATATACAGAATCA
AGGTTTACCTGGTAGTAAATCTCTGCCCAAAAGAGAACCAGAAGCGCATATGTAGTGAAG
AAAGCAAGACTTGGAATATCAAGTAAGATATGTTGCAGAATCTACAAGAAAAACCGAACG
ATACTTGCAAGTTTAGAAATTTCAAATAGACCATCAATGAGGCTAAATCACAATATCAAT
AGAAACACCAAACCTCTGGCTGCATGTTCTGTGCATCTCGCCGGAAGAGAAAAACCAG
   
Sequence Name 50-K029745-022-046-D04C-FL93
GenBank Accession LN507059 [GenBank]
Sequencing primer gene specific primer, FL93: 5'-CTTCTTTGTAGTTCGAGCTCTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K029745-022-046-D04C-FL93
ATGCAGCCAGAGGTTTGGTGTTTCTATTGATATTGTGATTTAGCCTCCTTGATGGTCTAT
TTGAAATTTCTAAACTTGCAAGTATCGTTCGGTTTTTCTTGTAGATTCTGCAACATATCT
TACTTGATATTCCAAGTCTTGCTTTCTTCACTACATATGCGCTTCTGGTTCTCTTTTGGG
CAGAGATTTACTACCAGGTAAACCTTGATTCTGTATATATTTTCAATTTAGTTTTTCCTG
CATTGTTGTTGATATTTACTCTATCTTGGCAGGCACGTGCTGTATCGACTGATGGACTGA
GGCCAAGTTTCTTCACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTACTCAAACTA
ACTACCTATTTGGCCTTTGTAATATCTGAGAAATTTTCAAAGTTTAGAGTTAACCTTGAA
CTTGGTGGACCTGTGGGCCTGAGCTTCTTATTGAATTTCCTTACGATCCATTTGTGTTGT
GGGTTCCTTAGCTTTAATCTTTCATTTGAATGTATTAATTTCAGATTGCTCTTTGGTTGG
TTTTGTGGTGGAAGCCTGTTCACCTTATGGTAATCATTTCTAAGATGTTCTTTGCAGGTA
TAGTTTGAAGTATAGATCATTTTACACAGAGATTGATTTTTTTAATGCACTCTATGTCTC
ATCTCTTGTGAATGTATATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGNTATTAC
CAATTTTTTTTTCATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG

Last Updated on 06.12.2018 16:11