Sequences from confirmation amplicon


Confirmation sequences for line 047A02 and hit At5g38200 are shown.
Line ID 047A02
Locus At5g38200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15256733
Primers used for PCR 02nj - 8474, show primer details
   
Sequence Name 58-K114716-0022-047-A02C-02nj
GenBank Accession LN509779 [GenBank]
Sequencing primer gene specific primer, 02nj: 5'-CACTATAAATTCGCCTTTTGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K114716-0022-047-A02C-02nj
TTACGTTTCACGTCACCATCAAATCTAACAAACACACATAAACTATTTTTTTTGTTATTT
GCTTTTTCATTTTCTAGGTACAAATCAAATGGGAACAATAGATATGGATCCAAAGTTGAA
TGATCTCTCTCAAATCCTCCCTCGTGTCCTCGTCGTATCTAGACGCACCGTTCGTAAAAA
CAAATTCGTCGATTTCGTTGGTTAGGACAAAACAAAACTCTACCTTCTTGTTCTGTTTTT
TTTTGTCTTAATCATGCAGGGTTCTGTTTTTTTTTGGCTTAAAATCGAAACTTTTGGATG
TTTCTTCTTCATAATGCAAATGGGTTCTCTTTATATTGTGGTTTTGTGAAATTGTGGGGT
TTTTCTTTTTCAGGTGAATACCATCTTGATTTGATAGTACGTTACGGATGTGTACCAGTG
ATTGTACCAAGAGTAACGGCTCATTTCTTCGTAAAAACCAACATGCAAGCTCCACCGGGT
GCAAAGCGGCAGCGGCGGCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAAATTTCC
   
Sequence Name 10-K114715-0022-047-A02C-8409
GenBank Accession LN490769 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114715-0022-047-A02C-8409
TTACAATTGAATAATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATGTTGG
TTTCTACGAAGAACTGAGCCGTTACTCTTGGTACAATCACTGGTACACATCCGTAACGTA
CTATCAAATCAAGATGGTATTCACCTGAAAAAGAAAAACCCCACAATTTCACAAAACCAC
AATATAAAGAGAACCCATTTGCATTATGAAGAAGAAACATCCAAAAGTTTCGATTTTAAG
CCAAAAAAAAACAGAACCATGCATGATTAAGACAAAAAAAAACAGAACAAGAAGGTAGAG
TTTTGTTTTGTCCTAACCAACGAAATCGACAAATTTGTTTTTACAAACGGTGCGTCTAGA
TACAACAAGGACACGAGGGAGGATTTGAGAGAGATCATTCAACTTTGGATCCATATCTAT
GGTTCCCATTTGATTTGTACCTAGAAAATGAAAAAGCAAATAACAAAAAAAATAGTTAAT
GTGTGTTTGTTAAATTTGATGGTGACGTGAAACGTTAACAAAGAGAGTTCTCGTGATAGA
GATAGAGAGAAGCAAAAGGCGAT

Last Updated on 06.12.2018 16:11