Sequences from confirmation amplicon


Confirmation sequences for line 047B07 and hit At1g58807 are shown.
Line ID 047B07
Locus At1g58807 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21783793
Primers used for PCR 60op - 8474, show primer details
   
Sequence Name 27-K114963-0022-047-B07C-60op
GenBank Accession LN498517 [GenBank]
Sequencing primer gene specific primer, 60op: 5'-TCAATATATCGGCTAGAGGAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114963-0022-047-B07C-60op
CTTTCTTATACTCTCTATATCGATGATTGTCCAAAGTTAAAGAAGCTTCCGGATGGGCTG
CAATTTATCTATTCATTAAAGAATTTGAAGATATCAGAAAGATGGAAGGAGAGATTGTCG
GAAGGAGGAGAAGAATATTACAAAGTCCAACACATTCCTTCTGTTGAATTCTACCATAGG
GTGTTGCATATCTTTAGGAGCGTCGGAGGTGATATAACTGGGAGACTCTTGATGAGATGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGC
   
Sequence Name 11-K114962-0022-047-B07C-8409
GenBank Accession LN491236 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114962-0022-047-B07C-8409
CCATTTACATTGAATATATCATCTCATCAAGAGTCTCCCAGTTATATCACCTCCGACGCT
CCTAAAGATATGCAACACCCTATGGTAGAATTCAACAGAAGGAATGTGTTGGACTTTGTA
ATATTCTTCTCCTCCTTCCGACAATCTCTCCTTCCATCTTTCTGATATCTTCAAATTCTT
TAATGAATAGATAAATTGCAGCCCATCCGGAAGCTTCTTTAACTTTGGACAATCATCGAT
ATAGAGAGTATGAAGAAAGGGCATAGAGCCTTGTTCTACTATCCACTCTTCCCATTCCTC
TAGCGAATATATTG

Last Updated on 06.12.2018 16:11