Sequences from confirmation amplicon


Confirmation sequences for line 047C08 and hit At3g28130 are shown.
Line ID 047C08
Locus At3g28130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10468180
Primers used for PCR CW70 - 8474, show primer details
   
Sequence Name 46-K104161-0022-047-C08C-CW70
GenBank Accession LN505853 [GenBank]
Sequencing primer gene specific primer, CW70: 5'-AAACCCTAACGATATCAAAATCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K104161-0022-047-C08C-CW70
ACATTACCCATAAAAGAAACATTAGATTAGATGTGTGGTTTTTCTTGTTTCACAAACAAG
TGTGTCTGAAGATTATTGATTCATATATACATATACCTTCCTAGGTAAAAAGAATCTCCA
AGAAAAATCGCTCCCATAATCACTGCTATTAAAATCGATAAAGGTCTGAATATAGCTAAG
TAAACAGGTCCTTTATTACGTACTGCCCACAGATGAATCGCGTAATATCCTGGATTAAAT
ATTCCCTGCGAAAAATAGCTATAGTATTGAAAAGAAGTTTAAGATATAATTAAGTTTTCT
AAAATATTAAAATTTACGTACCCCAACAACTATGCAAACTAATGTAATGTCAAAATGAAT
GATCCATATGCTCGGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCA
   
Sequence Name 46-K104162-0022-047-C08C-8409
GenBank Accession LN505854 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K104162-0022-047-C08C-8409
GCCATTTACAATCCGAGCATATGGATCATTCATTTTGACATTACATTAGTTTGCATAGTT
GTTGGGGTACGTAAATTTTAATATTTTAGAAAACTTAATTATATCTTAAACTTCTTTTCA
ATACTATAGCTATTTTTCGCAGGGAATATTTAATCCAGGATATTACGCGATTCATCTGTG
GGCAGTACGTAATAAAGGACCTGTTTACTTAGCTATATTCAGACCTTTATCGATTTTAAT
AGCAGTGATTATGGGAGCGATTTTTCTTGGAGATTCTTTTTACCTAGGAAGGTATATGTA
TATATGAATCAATAATCTTCAGACACACTTGTTTGTGAAACAAGAAAAACCACACATCTN
ATCTAATGGTTTCTTGTTATGTGGTTAATGTTGCAGTTTGGTCGGAGGGATTTTGATATC
TNTAGGGTT

Last Updated on 06.12.2018 16:11