Sequences from confirmation amplicon


Confirmation sequences for line 047D01 and hit At2g43880 are shown.
Line ID 047D01
Locus At2g43880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18173688
Primers used for PCR 01wc - 8474, show primer details
   
Sequence Name 14-K118356-0022-047-D01C-01wc
GenBank Accession LN492716 [GenBank]
Sequencing primer gene specific primer, 01wc: 5'-CTCCAGCGAACTACTGGGATATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118356-0022-047-D01C-01wc
CCGGATCTCGGTTACGGTGGCACCATTGATGCTAGAGGAGCTGGTTATTGGTCTTGTAGA
AAGAAAGGTAGTCATTGTCCTCAAGGTGCAAGGGTATGTTTACATCAAAACTAGCGGATT
TGATTTGTTCATTTATTAGCTAGTTATATTAATTTGATTTAATTATAATTGGACATGGCT
TCTTTTGCAGTCTATATCGTTCAGCTGGTGCAATAACGTTCTACTAAGTGGTCTATCGTC
GTTTAATAGCCAGAATATGCACGTCACGGTTCATCATTCTTCTAATGTTCGATGGAAAAC
TATTTGCTAAAATAGAACCACGTGGAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCACAAGATTATT
   
Sequence Name 14-K118355-0022-047-D01C-8409
GenBank Accession LN492715 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118355-0022-047-D01C-8409
TTTACATTCCACGTGGTCCTATTTTAGCAAATAGTTTTCCATCGAACATTAGAAGAATGA
TGAACCGTGACGTGCATATTCTGGCTATTAAACGACGATAGACCACTTAGTAGAACGTTA
TTGCACCAGCTGAACGATATAGACTGCAAAAGAAGCCATGTCCAATTATAATTAAATCAA
ATTAATATAACTAGCTAATAAATGAACAAATCAAATCCGCTAGTTTTGATGTAAACATAC
CCTTGCACCTTGAGGACAATGACTACCTTTCTTTCTACAAGACCAATAACCAGCTCCTCT
AGCATCAATGGTGCCACCGTAAACCGAGATCCGGTTTACTTTAGCGAAAAGAATCCAATA
ACCGGAATTACCTATATCCCAG

Last Updated on 06.12.2018 16:11