Sequences from confirmation amplicon


Confirmation sequences for line 047E09 and hit At3g44320 are shown.
Line ID 047E09
Locus At3g44320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15994784
Primers used for PCR RH04 - 8474, show primer details
   
Sequence Name 15-K018601-022-047-E09C-RH04
GenBank Accession LN492760 [GenBank]
Sequencing primer gene specific primer, RH04: 5'-GGTGAAATAATGACACTTCCACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K018601-022-047-E09C-RH04
TAGGATCATGTTCTTTAGTGTCTACTATGTCATTAAACAAGTAATCAGGATGTTCAGGGA
ACTCTCTACGCTTGCAGAACTGGTGCGCTGACAACACGAAACATCCACCTTCTACCGCAA
TGTGAATCATCGATGCTTGCCATTCCAACGAATAATCAGCAGTAGGTGCACAATAAATCT
CAATGCCTGCACACCAAAAAGCGCATATTTATTAGGAATCTTACTTTACAAGCCGGATAT
GTCAAGCTACATTGCATTGTAAAGCTGACCTTTAGCGTACAATGCAGTTCTGTAGAGGGG
CATCCTATTCTCCCAGCAAATAGCAGCACCGATTTTGCCAATAGGAGTGTCGTAAACGGG
GATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 16-K018601-022-047-E09C-8409
GenBank Accession LN493230 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018601-022-047-E09C-8409
CATGTAGATTTCCCGGACATGAAGCCATCCCCGTTTACGACACTCCTATTGGCAAAATCG
GTGCTGCTATTTGCTGGGAGAATAGGATGCCCCTCTACAGAACTGCATTGTACGCTAAAG
GTCAGCTTTACAATGCAATGTAGCTTGACATATCCGGCTTGTAAAGTAAGATTCCTAATA
AATATGCGCTTTTTGGTGTGCAGGCATTGAGATTTATTGTGCACCTACTGCTGATTATTC
GTTGGAATGGCAAGCATCGATGATTCACATTGCGGTAGAAGGTGGATGTTTCGTGTTGTC
AGCGCACCAGTTCTGCAAGCGTAGAGAGTTCCCTGAACATCCTGATTACTTGTTTAATGA
CATAGTAGACACTAAAGAACATGATCCTACTGTCTCCGGAGGTGGAAGTGTCATATTTTC
A
Primers used for PCR FL95 - 8474, show primer details
   
Sequence Name 29-K029743-022-047-E09C-8409
GenBank Accession LN499160 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K029743-022-047-E09C-8409
GCCATTTACAATTGAATATATCCTTGTGGGCATGACTTTACGGTGCTTACCTAAAAACTG
ACCTTGTGGACTGAAGAAAAGAGCTGTGCAATAGAGTGTATAACCATCCTTCTCTATAGC
CCCCATTACCAAGTGCACATTATTTTTCCCGGCCAACTCAGCCAATCTTTCCACTTCAGG
GCCTGTAGATTCATTACATCATTTACAAGAGTACCAAACATATGCAAGAAATAATCATTT
TGTGATCCAAGCTTTGAGAGTGCTCACTTAAACTAGAGCTATAACACTCATAGTGACAAA
AACAGAGAACTCTACATGAAGCAGAAACATGTTTTTCCTAAGAGTTAAGTTTACTTTGTT
CCAAATAAAATTTAACTGTCCTTAAGACTCCAACAACGGCCAGCT
   
Sequence Name 30-K029743-022-047-E09C-FL95
GenBank Accession LN499595 [GenBank]
Sequencing primer gene specific primer, FL95: 5'-TTAGCTGCTGTTGTTGGAGTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K029743-022-047-E09C-FL95
CAAGTAACTTAACTCTTAGAAAAACATGTTTCTGCTTCATGTAGAGTTCTCTGTTTTTGT
CACTATGAGTGTTATAGCTCTAGTTTAAGTGAGCACTCTCAAAGCTTGGATCACAAAATG
ATTATTTCTTGCATATGTTTGGTACTCTTGTAAATGATGTAATGAATCTACAGGCCCTGA
AGTGGAAAGATTGGCTGAGTTGGCCGGGAAAAATAATGTGCACTTGGTAATGGGGGCTAT
AGAGAAGGATGGTTATACACTCTATTGCACAGCTCTTTTCTTCAGTCCACAAGGTCAGTT
TTTAGGTAAGCACCGTAAAGTCATGCCCACAAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
Primers used for PCR DC82 - 8474, show primer details
   
Sequence Name 04-K032598-022-047-E09C-8409
GenBank Accession LN487806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K032598-022-047-E09C-8409
CCGTTACGACACTCCTATTGGCAAAATCGGTGCTGCTATTTGCTGGGAGAATAGGATGCC
CCTCTACAGAACTGCATTGTACGCTAAAGGTCAGCTTTACAATGCAATGTAGCTTGACAT
ATCCGGCTTGTAAAGTAAGATTCCTAATAAATATGCGCTTTTTGGTGTGCAGGCATTGAG
ATTTATTGTGCACCTACTGCTGATTATTCGTTGGAATGGCAAGCATCGATGATTCACATT
GCGGTAGAAGGTGGATGTTTCGTGTTGTCAGCGCACCAGTTCTGCAAGCGTAGAGAGTTC
CCTGAACATCCTGATTACTTGTTTAATGACATAGTAGACACTAAAGAACATGATCCTACT
GTCTCCGGACGTGGAAGTG
   
Sequence Name 04-K032598-022-047-E09C-DC82
GenBank Accession LN487807 [GenBank]
Sequencing primer gene specific primer, DC82: 5'-GGTGAAATAATGACACTTCCACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K032598-022-047-E09C-DC82
TATGTCATTAAACAGTAATCAGGATGTTCAGGGAACTCTCTACGCTTGCAGAACTGGTGC
GCTGACAACACGAAACATCCACCTTCTACCGCAATGTGAATCATCGATGCTTGCCATTCC
AACGAATAATCAGCAGTAGGTGCACAATAAATCTCAATGCCTGCACACCAAAAAGCGCAT
ATTTATTAGGAATCTTACTTTACAAGCCGGATATGTCAAGCTACATTGCATTGTAAAGCT
GACCTTTAGCGTACAATGCAGTTCTGTAGAGGGGCATCCTATTCTCCCAGCAAATAGCAG
CACCGATTTTGCCAATAGGAGTGTCGTAAACGGGGATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11