Sequences from confirmation amplicon


Confirmation sequences for line 047E12 and hit At1g53560 are shown.
Line ID 047E12
Locus At1g53560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19984922
Primers used for PCR FP17 - 3144, show primer details
   
Sequence Name 34-K032925-0022-047-E12C-3144
GenBank Accession LN501310 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K032925-0022-047-E12C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGGTTTAAACTATCAGTGTTTCCCACCATGGTGTGCA
AATAAATACATTGTTCATCAAGTATCTTGAACTTGTATAGGAGAAACCTTTTTGGTGTTT
TTAATGGGGATGATTTAAGTGTGGGAGTGCTTGTTGATTTAAGTGTGCAAATAAATACAT
TCTTCATCAAGTATCTTGAGCTAGTAACTAAACAGAGAAATATACAAAAACTTAAAAAGA
AGCACAAGAAAACAAAGAGAAACAGAAAAAAAAGAAGAAGATAGATAAATGTGATTAGTT
ATGTTTGGGAGAAAGTAAACACTTAGATAAGCGTCACATTCTCTAAAAAATTTGAAAATG
TGTAAAATAATCTAGCATAAGTAAACTGTTTAAGTATTGGTACTTGAAGTGTTACTCTTG
TAATAATGTTCCTCTCGAGATTGTAGTAGTAGATGAAAGGATACCTTGCATCAGTACTTG
TGTTAGCACTAGAGATTGGGTACAACAAATTTCCACCAC
   
Sequence Name 34-K032925-0022-047-E12C-FP17
GenBank Accession LN501311 [GenBank]
Sequencing primer gene specific primer, FP17: 5'-AAGTGGTGAAATTTTGTTGTACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K032925-0022-047-E12C-FP17
GATGCAGGTATCCTTTCATCTACTACTACAATCTCGAGAGGAACATTATTACAAGAGTAA
CACTTCAAGTACCAATACTTAAACAGTTTACTTATGCTAGATTATTTTACACATTTTCAA
ATTTTTTAGAGAATGTGACGCTTATCTAAGTGTTTACTTTCTCCCAAACATAACTAATCA
CATTTATCTATCTTCTTCTTTTTTTTCTGTTTCTCTTTGTTTTCTTGTGCTTCTTTTTAA
GTTTTTGTATATTTCTCTGTTTAGTTACTAGCTCAAGATACTTGATGAAGAATGTATTTA
TTTGCACACTTAAATCAACAAGCACTCCCACACTTAAATCATCCCCATTAAAAACACCAA
AAAGGTTTCTCCTATACAAGTTCAAGATACTTGATGAACAATGTATTTATTTGCACACCA
TGGTGGGAAACACTGATAGTTTAAACCGACAATCTGATCCCCGGGTACCCTGTCCTCTCC
AAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCG
TCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGA
ACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG
Primers used for PCR KM15 - 8474, show primer details
   
Sequence Name 64-K032606-0022-047-E12C-KM15
GenBank Accession LN511125 [GenBank]
Sequencing primer gene specific primer, KM15: 5'-CTCTTCCAGAAGCAAAAGCTTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K032606-0022-047-E12C-KM15
CTGTTTCTCATTAGTACTACACAAAAAGTTAAAAGTAAATGTGTAACTAAATGTTGCAGG
TTACCCTGTTTCTCAAGGAAGACCTGCAAGACAAAACCGTCTTCCTTGTTGTGGTCTTGG
CCTCGGTTGGTTCTTGTAAGTTCTTGTTTTTCCCGAGCAATCTCAAATTAGACTTTGTTT
CAATACATTTTGTCTGAAAAAACTCAATCCTTTTTTGGTAAAGGTTCATCATTGGTTTCC
TCCTTGGTGCAATTCCATGGTATGTAGGCGTATTCATTCTAGTATGTGCTAGGATCGATC
CACGAGAGAAACCAGGATACGTAGCTTGTACCATTGCAGTGAGTCTTTCACTAAAAGATA
ATCGTGTTTAATACATTAATCTTGTGATTTAATCACTTTTTAACACTTTATACTTTCACA
GGCTGTTGTTGCTACAATTACCATAGTTTTTGGTGTCATGGGAGGAAGAGTAAATGAGTA
AATTAGAATAATGTGTTTATTGCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 64-K032606-0022-047-E12C-8409
GenBank Accession LN511124 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K032606-0022-047-E12C-8409
CCATTTACTGCAATAAACACATTATTCTAATTTACTCATTTACTCTTCCTCCCATGACAC
CAAAAACTATGGTAATTGTAGCAACAACAGCCTGTGAAAGTATAAAGTGTTAAAAAGTGA
TTAAATCACAAGATTAATGTATTAAACACGATTATCTTTTAGTGAAAGACTCACTGCAAT
GGTACAAGCTACGTATCCTGGTTTCTCTCGTGGATCGATCCTAGCACATACTAGAATGAA
TACGCCTACATACCATGGAATTGCACCAAGGAGGAAACCAATGATGAACCTTTACCAAAA
AAGGATTGAGTTTTTTCAGACAAAATGTATTGAAACAAAGTCTAATTTGAGATTGCTCGG
GAAAAACAAGAACTTACAAGAACCAACCGAGGCCAAGACCACAACAAGGAAGACGGTTTT
GTCTTGCAGGTCTTCCTTGAGAAACAGGGTAACCTGCAACATTTAGTTACACATTTACTT
TTAACTTTTTGTGTAGTACTAATGTAGAAACAGAGAGAGCTTCCCAACATTGAATATAGT
TTTAA

Last Updated on 06.12.2018 16:11