Sequences from confirmation amplicon


Confirmation sequences for line 048G03 and hit At3g05750 are shown.
Line ID 048G03
Locus At3g05750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1704878
Primers used for PCR 01fr - 8474, show primer details
   
Sequence Name 36-K111470-0022-048-G03C-01fr
GenBank Accession LN502270 [GenBank]
Sequencing primer gene specific primer, 01fr: 5'-TACTGTCGAAAGTTCGGTCTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K111470-0022-048-G03C-01fr
ATTCCTCTGTATAGTTGGCTGAGATTGAGTACATATTTAGAGTCCATAGAAATAATTCAA
TTGCTGTGTTATGTAGATGGTAGAAATGGAGGCTGTTGAGAGGAAACGATCCAGAGGAGG
TTTTCTAAATATGTTTGATTGGCCTGGTAAATCCCGTAAGAAGCTCTTCTCCTCTAGCAG
CAGCAGCTCGAAACTCTCCGGTAATGTCTACTTTAATGCTCTATCAGTTTTGGTTTAAGT
TCAAATGGTTCTTGTCTTGAAGCCCTTGGTTGGTTTCTCCACCGGGTGCAAAGCGGCAGC
GGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 20-K111469-0022-048-G03C-8409
GenBank Accession LN495265 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K111469-0022-048-G03C-8409
ATTTCATTGAATTATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGAAACCAACCAAG
GGCTTCAAGACAAGAACCATTTGAACTTAAACCAAAACTGATAGAGCATTAAAGTAGACA
TTACCGGAGAGTTTCGAGCTGCTGCTGCTAGAGGAGAAGAGCTTCTTACGGGATTTACCA
GGCCAATCAAACATATTTAGAAAACCTCCTCTGGATCGTTTCCTCTCAACAGCCTCCATT
TCTACCATCTACATAACACAGCAATTGAATTATTTCTATGGACTCTAAATATGTACTCAA
TCTCAGCCAACTATTACAGATGGAATTTTTATTTGGTTTTTCAATCAAAAGGACAAAAAA
AGACCGAACTTTCGACAGTA

Last Updated on 06.12.2018 16:11