Genotyping and primer details


Genotyping and primer details for 048G03 - At3g05750
Line ID 048G03
Locus At3g05750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1704878 of line 048G03 Chr3:1704878
   
Insertion allele amplicon 8474 - 01fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01fr: 5'-TACTGTCGAAAGTTCGGTCTTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1704545
   
wt amplicon (over InsPos) 09gl - 01fr
wt amplicon start Chr3:1704545
wt amplicon end Chr3:1705450
wt amplicon size 906 bp
Locus specific primer 01fr: 5'-TACTGTCGAAAGTTCGGTCTTTTT-3'
2nd locus specific primer 09gl: 5'-CATCATAGTCACTGCGGAGATTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11