Sequences from confirmation amplicon


Confirmation sequences for line 048G11 and hit At5g53990 are shown.
Line ID 048G11
Locus At5g53990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21915766
Primers used for PCR ZZ97 - 8474, show primer details
   
Sequence Name 57-K023492-022-048-G11C-8409
GenBank Accession LN509289 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K023492-022-048-G11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAAATCTACATGAAACTTTGCAGAATATAGTCA
ACGATACAAATCTTGAATGAAACAATTTCATTATAAAGAAAATATGTCACAAATAAGGTA
AAATAAGGAACTAGACCATGAAGTCGTTATTAGTGTCTTGTCTCTTCCTCAAAGTTGTAA
TAGATTTGTCTTTGTTCAATTTTATTTTATTTCTATAAATTCATCAACTCGTTTATGAAA
AAAAAAACTGCTTGTTGACTCTAGTGATCCAAATCCACAAAGCAAGAATGCGATTTTTCT
TTCATATATAAAAGACAT
   
Sequence Name 58-K023492-022-048-G11C-ZZ97
GenBank Accession LN509584 [GenBank]
Sequencing primer gene specific primer, ZZ97: 5'-ATATCTTGGTCAATCCCATGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K023492-022-048-G11C-ZZ97
TATATGAAAGAAAAATCGCATTCTTGCTTTGTGGATTTGGATCACTAGAGTCAACAAGCA
GTTTTTTTTTTCATAAACGAGTTGATGAATTTATAGAAATAAAATAAAATTGAACAAAGA
CAAATCTATTACAACTTTGAGGAAGAGACAAGACACTAATAACGACTTCATGGTCTAGTT
CCTTATTTTACCTTATTTGTGACATATTTTCTTTATAATGAAATTGTTTCATTCAAGATT
TGTATCGTTGACTATATTCTGCAAAGTTTCATGTAGATTTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATGGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
Primers used for PCR FL99 - 8474, show primer details
   
Sequence Name 59-K029745-022-048-G11C-8409
GenBank Accession LN509891 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K029745-022-048-G11C-8409
GCCATTTACAATTGAAACCGGTTAATAATCCAGGACTAACCAAAACCTCCTTTAATTTAG
CGTGGTTCCTCCTCACCAGATTCCCTAACTCACTATCTTTGTCCATCACCGAGGTGATCG
CAACACTTAAGCTCTCTTTCGAGAACCATCCTGTTTCTTCTCTTTTCACTTCCACCGAAA
CCTCGAGTTCCTCACTCATCAATCTAGTGTTGAGAATTTGATCACACAAATATGGAAGCA
AGACTATTTGACAATCACTCACTAGAGACTCCCACATTGATCCAAACCCACAATGGGTCA
CAAAGCAGCCTACTGATGGATGAGCCAATATCAATGGTTGCTGCACCCATTCTCCCCAAA
CTACTCCATGATTCTTCACCCTCTCCTCAAACCCTTCTGGCAACGCTTCTTGGATCGTCT
TTGCGCCTTTTGGTGGTTTTACCGCTACGAGAAACGGTAAACCAGTGAGCTCCATTCCTA
AACAGAGTTCTT
   
Sequence Name 60-K029745-022-048-G11C-FL99
GenBank Accession LN510155 [GenBank]
Sequencing primer gene specific primer, FL99: 5'-GAAGGATCAATTCCAAGAACTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K029745-022-048-G11C-FL99
TAGCGGTAAAACCACCAAAAGGCGCAAAGACGATCCAAGAAGCGTTGCCAGAAGGGTTTG
AGGAGAGGGTGAAGAATCATGGAGTAGTTTGGGGAGAATGGGTGCAGCAACCATTGATAT
TGGCTCATCCATCAGTAGGCTGCTTTGTGACCCATTGTGGGTTTGGATCAATGTGGGAGT
CTCTAGTGAGTGATTGTCAAATAGTCTTGCTTCCATATTTGTGTGATCAAATTCTCAACA
CTAGATTGATGAGTGAGGAACTCGAGGTTTCGGTGGAAGTGAAAAGAGAAGAAACAGGAT
GGTTCTCGAAAGAGAGCTTAAGTGTTGCGATCACCTCGGTGATGGACAAAGATAGTGAGT
TAGGGAATCTGGTGAGGAGGAACCACGCTAAATTAAAGGAGGTTTTGGTTAGTCCTGGAT
TATTAACCGGTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11