Sequences from confirmation amplicon


Confirmation sequences for line 049A06 and hit At5g41750 are shown.
Line ID 049A06
Locus At5g41750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16696179
Primers used for PCR EP72 - 8474, show primer details
   
Sequence Name 15-K103223-0022-049-A06C-8409
GenBank Accession LN492932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103223-0022-049-A06C-8409
TCGGACTGAGCATTTACATTGAATTATCCTATAGTTTAACACAACAAATGAGAAAATATC
ATATTTATTTAAATTGAATCATTAGAAGCACAAGAAAGAATAACCAAATACCAACCTGGA
TTCCTCCCCAGAGCTTCTTGAGCTTGCTGCTTGGCATACGAATTTTGACGAGATGTTCCG
GGTTGAATCTCTGAGGAAGACTTTTTCTTGGGTAATTTTGCCAATGTAGTAACCTAACAG
GAGGTATATACTCCATATCTTCTGGTATTTGCAAAGTACCTTCACTATTGAAAGAGTCTC
TATAGATTCTTAGGAATTGGAGATTACGCATTCCTTCAAAAGCCCCCTTACCTACCGAAA
CCTCTTCGATATTGGATGTATCAAATGATATACCTTTGACAGATTCAGTACCCTACAAAA
TATTCCCAAAGAAGACTGATGAGAT
   
Sequence Name 63-K103230-0022-049-A06C-EP72
GenBank Accession LN510964 [GenBank]
Sequencing primer gene specific primer, EP72: 5'-AATCTCATCAGTCTTCTTTGGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103230-0022-049-A06C-EP72
CATTTGATACATCCATATCGAAGAGGTTTCGGTAGGTACGGGGGCTTTTGAAGGAATGCG
TAATCTCCAATTCCTAAGAATCTATAGAGACTCTTTCAATAGTGAAGGTACTTTGCAAAT
ACCAGAAGATATGGAGTATATACCTCCTGTTAGGTTACTACATTGGCAAAATTACCCAAG
AAAAAGTCTTCCTCAGAGATTCAACCCGGAACATCTCGTCAAAATTCGTATGCCAAGCAG
CAAGCTCAAGAAGCTCTGGGGAGGAATCCAGGTTGGTATTTGGTTATTCTTTCTTGTGCT
TCTAATGATTCAATTTAAATAAATATGATATTTTCTCATTTGTTGTGTTAAACTATAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11