Sequences from confirmation amplicon


Confirmation sequences for line 049C12 and hit At3g49940 are shown.
Line ID 049C12
Locus At3g49940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18514756
Primers used for PCR CL51 - 8474, show primer details
   
Sequence Name 40-K032925-022-049-C12C-8409
GenBank Accession LN503613 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K032925-022-049-C12C-8409
TTTACAATTGAATATCTAACGTTGTCGTCTAAACGTTGTCGTTTTGCTATACGAAGCTTG
TGGGAGAACTGTGAATCCGGTGAACGGAGCCGTCGGATTGTTGTGGACGGGGAATTGGAA
TGTTTGTCAAGCGGCGGTTGAGACGGTGCTTCGTGGTGGTTCTTTAAAACCAATACCGGA
GCTTCTTAACGGCGGTGGATTCGCCGGGTTTCCGTCTCCTACTTCCGACGAAGCTTCGGA
GATCTGTACGGAAATGTTGAATCTACGAAAAGCTGATGATTCCGGTGATCGGAACATTTA
TCATCACTGCCGATTCTCAAGCTCTAGATCTAGATCAAGATCAACAGCTTCTCCGCCGAA
ACGGAAACGATTATCGTCGGAACAACAACCTTCGTCGGAGCTTGATCTCTCTCTTATTCC
TATTTATCCGATTAAAACCTTGCCGTTTAAGGAAGATACACCGTCGATGTACTCGGAGGA
GTCTGTTACCACGGTTTCGTTTCAAAACAACAACGCCGGTGATCGGTACGTACGCTGCGG
CGGAGGAGGAGGAGGAGCAACGACAAAGTTGCTCAATCTCT
   
Sequence Name 40-K032925-022-049-C12C-CL51
GenBank Accession LN503614 [GenBank]
Sequencing primer gene specific primer, CL51: 5'-AAGAGATTGAGCAACTTTGTCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K032925-022-049-C12C-CL51
GGGTGGTGGAGCAACGACAAAGTTGCTCAATCTCTTAGNGGNNNNNGAGAGAGATCNAGC
TCCGACGAAGGTTGTTGTTCCGACGATAATCGTTTCCGTTTCGGCGGAGAAGCTGTTGAT
CTTGATCTAGATCTAGAGCTTGAGAATCGGCAGTGATGATAAATGTTCCGATCACCGGAA
TCATCAGCTTTTCGTAGATTCAACATTTCCGTACAGATCTCCGAAGCTTCGTCGGAAGTA
GGAGACGGAAACCCGGCGAATCCACCGCCGTTAAGAAGCTCCGGTATTGGTTTTAAAGAA
CCACCACGAAGCACCGTCTCAACCGCCGCTTGACAAACATTCCAATTCCCCGTCCACAAC
AATCCGACGGCTCCGTTCACCGGATTCACAGTTCTCCCACAAGCTTCGTATAGCAAAACG
ACAACGTTTAGACGACAACGTTAAGATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCC

Last Updated on 06.12.2018 16:11