Sequences from confirmation amplicon


Confirmation sequences for line 049H07 and hit At4g36870 are shown.
Line ID 049H07
Locus At4g36870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17370778
Primers used for PCR JU15 - 8474, show primer details
   
Sequence Name 33-K021056-022-049-H07C-8409
GenBank Accession LN500816 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K021056-022-049-H07C-8409
GATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAAAATTGATTCATA
TTTTTGATTATTTTGGTAGAGTTTGATTGCGCAATCCTACCATCATTTGTTTAAAATTAA
AATTAATTTAATCATTTTGCTCGTGTGACGGTCGTATTAGAAAGTATATTGAATATTTTG
ATGCTTAGCGTTTGGTGGAATCGATTAATCAAAGAAAATGCATTAAATAATTACGATTAA
TTATGTAATTGGCGTTTCCTTCTCCATATAAGCTACTCTCCAGTTACGTCCTAAAGCTAA
CCCTTGACGTTACTTATTTGTTTACCCATAATTGAATTATCTACTTTTTAGTTGACCTTG
CTTTTTTCTCACTAGAAATAAATATTTTAGTTTATGCTCGAGGATACGATAAAATGTGCT
GTTGGTAGGATTGCGCAAACATTGAAGTATATTCTCTGGTCAATAATTTGTCTATATAAT
ATCTTATTTTCTTATTTTGTCTCGTTTAAAGGTAACTAATTAATCTCATTAGTTTATTTA
T
   
Sequence Name 34-K021056-022-049-H07C-JU15
GenBank Accession LN501241 [GenBank]
Sequencing primer gene specific primer, JU15: 5'-TATGTCGAAAGAGTTCACAACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K021056-022-049-H07C-JU15
TGCATTTGCTCGCAGTAATGGTTGTACCGTCGGTCCACCTGCCAACGAAACAAAACTATA
CATTATACAATCCATAAATATCAATATTTGCCTTTTTCTTTTAATTAATGTTATTGTTAA
CTCGTGTGTGTTGTGTTATATGTTCACGTGATTATTATTTAGTGTAGATTTTATCACGGG
AACCTATGCTTGTGTACGAGCATCATCTAATAAATAAACTAATGAGATTAATTAGTTACC
TTTAAACGAGACAAAATAAGAAAATAAGATATTATATAGACAAATTATTGACCAGAGAAT
ATACTTCAATGTTTGCGCAATCCTACCAACAGCACATTTTATCGTATCCTCGAGCATAAA
CTAAAATATTTATTTCTAGTGAGAAAAAAGCAAGGTCAACTAAAAAGTAGATAATTCAAT
TATGGGTAAACAAATAAGTAACGTCAAGGGTTAGCTTTAGGACGTAACTGGAGAGTAGCT
TATATGGAGAAGGAAACGCCCATTACATAATTAATCGTAATTATTTAATGCATTTTCTTT
GATTAATCGATTCCACCAAACGCTAAGCATCAAAATATTCAATATACTTTCTAATACGAC
CGTCCCACGAGCAAAATGATTAAATTAATTTTAATTTTAAACAAATGATGGTAGGATTGC
GCAATCAAACTCTACCAAAATAATCAAAAATATGAATCAATT
Primers used for PCR FM06 - 3144, show primer details
   
Sequence Name 90-K029207-022-049-H07C-3144
GenBank Accession LN514997 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K029207-022-049-H07C-3144
CATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGG
ACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTG
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGAAACAAAA
GACCAATTCAGTCATATCAGGTTTCTTGTTTCTTTGTCATAACTCTTGGATCAATTTATG
AATCTTATCGAAATGTCACGAAAACAATTTTACGTAAAATGTATGAACTAATATCACTGT
AGAGTATAGACAAAGTCAAAATCAATATTAAAAGTTAATATGAACATACCTCTTCAAGCA
TGGTGAGTAGTTTCACTTTCCTTCTTTGATGCTCAATCCGATCAGACGCCGATAAAGGAG
GATGCTCCTTGTTTGCTCCGGCCGACGAAGGAGAGCTGCCACCACCATCACCACCGCAAG
TATTAGGGTTTGAGCTGTTCCCAAGTTTGTTCTTCT
   
Sequence Name 91-K029207-022-049-H07C-FM06
GenBank Accession LN515081 [GenBank]
Sequencing primer gene specific primer, FM06: 5'-GAAGAAGAACAAACTTGGGAACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K029207-022-049-H07C-FM06
CGGTGGTGATGGTGGTGGCAGCTCTCCTTCGTCGGCCGGAGCAAACATGGAGCATCCTCC
TTTATCGGCGTCTGATCGGATTGAGCATCAAAGAAGGAAAGTGAAACTACTCACCATGCT
TGAAGAGGTATGTTCATATTAACTTTTAATATTGATTTTGACTTTGTCTATACTCTACAG
TGATATTAGTTCATACATTTTACGTAAAATTGTTTTCGTGACATTTCGATAAGATTCATA
AATTGATCCAAGAGTTATGACAAAGAAACAAGAAACCTGATATGACTGAATTGGTCTTTT
GTTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAAA
CACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAAAGAAAGGGTCTTGCCAAGGATATTGGGATTGT
GCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTT
GGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGG

Last Updated on 06.12.2018 16:11