Sequences from confirmation amplicon


Confirmation sequences for line 050A12 and hit At1g71170 are shown.
Line ID 050A12
Locus At1g71170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26831519
Primers used for PCR 04tv - 8474, show primer details
   
Sequence Name 37-K117029-0022-050-A12C-04tv
GenBank Accession LN502756 [GenBank]
Sequencing primer gene specific primer, 04tv: 5'-AAAGGTTTGAATATAGCTGCTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117029-0022-050-A12C-04tv
GACCTTTGATGGTCGTGTTTAGATTATAGGCTAAGTGGCTATAGAGAAATAGTTATAGGA
GGTAGAAGTTGATAATCCAATTGGAAACTAATGTTACACACTCTTGTAACTATAGTTCTC
GAGATTGGTGAGACTCCATGTTCTTATTAGCAACTTCTAACCAAATGGAAAACAAGCTTG
CCTAGTTAACACTAGTTTTATCAGGATAAGCCGTTAAGTCTCCTAATAACATCGACGACG
CCTTGAAATCCTAAATCTACATGTCCGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGGTTATTAT
   
Sequence Name 05-K117028-0022-050-A12C-8409
GenBank Accession LN488521 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117028-0022-050-A12C-8409
CCGGACATGTAGATTTAGGATTTCAAGGCGTCGTCGATGTTATTAGGAGACTTAACGGCT
TATCCTGATAAAACTAGTGTTAACTAGGCAAGCTTGTTTTCCATTTGGTTAGAAGTTGCT
AATAAGAACATGGAGTCTCACCAATCTCGAGAACTATAGTTACAAGAGTGTGTAACATTA
GTTTCCAATTGGATTATCAACTTCTACCTCCTATAACTATTTCTCTGTTACCCACTTAGC
CTATAATCTTAAACACGACCATCAAAGGTCTTACATTATACAAGTCACCAAAGTCTCTTC
AACTGAAACGTTGGAGCAGCTATTCACAAACCTTT

Last Updated on 06.12.2018 16:11