Sequences from confirmation amplicon


Confirmation sequences for line 050C07 and hit At2g20270 are shown.
Line ID 050C07
Locus At2g20270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8739030
Primers used for PCR 02ve - 8474, show primer details
   
Sequence Name 66-K118051-0022-050-C07C-02ve
GenBank Accession LN511701 [GenBank]
Sequencing primer gene specific primer, 02ve: 5'-ACAAGAAAGAACAAATGGTTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K118051-0022-050-C07C-02ve
GATACTAGAAGATGCAACATAATAGTTTCTAACAAATAGAAGCATACTGCCTATGATTCA
AAGGCAGGAAAGAATCTTCAATTTGAAAAATTTAAGCCATTAGAAGTACTAGCCTACCCG
TTGATTCTTCATCAACAAGTCATTGGTCCAAGGAAAACTACAGTGGAAGTAAATTTTTCA
CATAAACAAGACACAGAATCTCTGAAATGTAAAGCTATCTTAGGCCATTAGAAACCCCCC
TAACCATCATCAATCCTCCATCAAAATCTAATCAAAAAACATACAAACCAATTCAAGCAA
GCAGCCAAATGAACCAGAAGATAAACAAGAAGAAGTTGGAAACAAGTATTAATTTCATCC
AGTCAAAATGCTGAAACATTAGGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATATCCGCACGATTATT
   
Sequence Name 30-K118050-0022-050-C07C-8409
GenBank Accession LN499905 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118050-0022-050-C07C-8409
TTGCCTAATGTTTCAGCATTTTGACTGGATGAAATTAATACCTGTTTCCAACTTCTTCTT
GTTTATCTTCTGGTTCATTTGGCTGCTTGCTTGAATTGGTTTGTATGTTTTTTGATTAGA
TTTTGATGGAGGATTGATGATGGTTAGGGGGGTTTCTAATGGCCTAAGATAGCTTTACAT
TTCAGAGATTCTGTGTCTTGTTTATGTGAAAAATTTACTTCCACTGTAGTTTTCCTTGGA
CCAATGACTTGTTGATGAAGAATCAACGGGTAGGCTAGTACTTCTAATGGCTTAAATTTT
TCAAATTGAAGATTCTTTCCTGCCTTTGAATCTTAGACAGTATGCTTCTATTTGTTAGAA
ACTATTATGTTGCTATCTTCTTAGTTATCTTTTCTCGTGTCAAGATTTGGTGACTTTCCA
AAACCATTTGTTCTTTCTTGT

Last Updated on 06.12.2018 16:11