Sequences from confirmation amplicon


Confirmation sequences for line 051A05 and hit At3g54400 are shown.
Line ID 051A05
Locus At3g54400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20142442
Primers used for PCR CW48 - 8474, show primer details
   
Sequence Name 71-K104127-0022-051-A05C-CW48
GenBank Accession LN512550 [GenBank]
Sequencing primer gene specific primer, CW48: 5'-GTTCGTATTTCGGTTTTGGTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K104127-0022-051-A05C-CW48
AAATATCACGTCCAATACTAACCATTTTTTTTCTTTCGATTTGAAACATTTATGTATGTT
CTGATTAGATCTTGTTTTGAACAAAGTAAAAATGCGAATTATTTGTTAGTATAATTTAAA
TGTATTTTTTGTATATCGATTTTCCCGGTTTATTCAGTACAAAAGTATCGTTAAGTATGA
ATGTATGATATGATTCAAAAAAATTACGTTGAGTATAGTTTTCATTTTTCAAGTCCATCC
AGAGAATGCATGCACATATCTAATTTATACGGAGGGTATAACTATAAAGCAATATTTAGT
TACCTGTTTACACTGAGGAGCTTCGCATTGAAGAGTACGAGAGGAGCTTGACTTGGAAGG
GTCAAAGAGAACAGAGGAAGAACAGCCAACGCAGCCTGAACAAGGAATCCAAGCAGCGTC
ATTGCTAGTGTCAAGAGCCACGAGCATGGGCTGAGCCGGTGTCCCGATGTTAGCCCTCAC
GATGTAAGTCGGGCTCTGAACGATGGCCCGACCAGAGGCGATTGGAACTGATGATTTCCT
AACGCCAGCGAGGCTTGACAAGTATAGGAAACGAGCCTTATCTTGAAGAAGTGTATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 84-K104128-0022-051-A05C-8409
GenBank Accession LN514424 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K104128-0022-051-A05C-8409
TTCTTCAGATAAGGCTCGTTTCCTATACTTGTCAAGCCTCGCTGGCGTTAGGAAATCATC
AGTTCCAATCGCCTCTGGTCGGGCCATCGTTCAGAGCCCGACTTACATCGTGAGGGCTAA
CATCGGGACACCGGCTCAGCCCATGCTCGTGGCTCTTGACACTAGCAATGACGCTGCTTG
GATTCCTTGTTCTGGCTGCGTTGGCTGTTCTTCCTCTGTTCTCTTTGACCCTTCCAAGTC
AAGCTCCTCTCGTACTCTTCAATGCGAAGCTCCTCAGTGTAAACAGGTAACTAAATATTG
CTTTATAGTTATACCCTCCGTATAAATTAGATATGTGCATGCATTCTCTGGATGGACTTG
AAAAATGAAAACTATACTCAACGTAATTTTTTTGAATCATATCATACATTCATACTTAAC
GATACTTTTGTACTGAATAAACCGGGAAAATCGATATACAAAAAATACATTTAAATTATA
CTAACAAATAATTCGCATTTTTACTTTGTTCAAAACAAGATCTAATCAGAACATACATAA
ATGTTTCAAATCGAAAGAAAAAAAATGGTTAGTATTTGGACTTGATTATTTTTAACAAAA
CAAGAAATTTAAAACCAAAACC

Last Updated on 06.12.2018 16:11