Sequences from confirmation amplicon


Confirmation sequences for line 051G05 and hit At5g49980 are shown.
Line ID 051G05
Locus At5g49980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20335424
Primers used for PCR 27ke - 3144, show primer details
   
Sequence Name 56-K113379-0022-051-G05C-27ke
GenBank Accession LN509162 [GenBank]
Sequencing primer gene specific primer, 27ke: 5'-TAAGCTCTGGACAGTTCTCTGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K113379-0022-051-G05C-27ke
CAAAGTGAGAATAGATTCTAGTTTCCTACAACCCTCGGAGATTGCTTGGAGGCCTAATTC
AGAGACAGGACCTTCACTGTCTTCCCGAGGATCAAAAGGGAAGATCCTGAGTTCACGGAG
TTCCTTGCAAGTGGCTGCAACTGCCTGTAGTCCTTCATCACATATTGAATCAAGGGCCTG
CATTGTAAAGTTCAAGTCATGCATTTTACTCAAGTTCCTTACACAACTAATATCAAGTTT
ATTCATTCTATCCATCTAACACAAACAATACGGTCACTAGAGTTAAGTCACATATAACTT
AATAAAGCCATGAAGACATAGATCAAGAAGGATACATACCCAGAACACCTGGAGTTTGTG
GCAATTGAGTATGATGGGCTTGAACATGTCAGGAGAAATGTTAGCATAACTGAAGTTCAG
GGAAGTTCACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCC
TGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTG
GGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC
   
Sequence Name 18-K113377-0022-051-G05C-3144
GenBank Accession LN494384 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K113377-0022-051-G05C-3144
TTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGAACTTCCCTGAACTTCA
GTTATGCTAACATTTCTCCTGACATGTTCAAGCCCATCATACTCAATTGCCACAAACTCC
AGGTGTTCTGGGTATGTATCCTTCTTGATCTATGTCTTCATGGCTTTATTAAGTTATATG
TGACTTAACTCTAGTGACCGTATTGTTTGTGTTAGATGGATAGAATGAATAAACTTGATA
TTAGTTGTGTAAGGAACTTGAGTAAAATGCATGACTTGAACTTTACAATGCAGGCCCTTG
ATTCAATATGTGATGAAGGACTACAGGCAGTTGCAGCCACTTGCAAGGAACTCCGTGAAC
TCAGGATCTTCCCTTTTGATCCTCGGGAAGACAGTGAAGGTCCTGTCTCTGAATTAGGCC
TCCAAGCAATCTCCGAGGGTTGTAGGAAACTAGAATCTATTCTCTACTTTTGCCAGCGCA
TGACTAATGCCGCTGTGATAGCCATGTCAGAGAAC

Last Updated on 06.12.2018 16:11