Sequences from confirmation amplicon


Confirmation sequences for line 052D06 and hit At2g46740 are shown.
Line ID 052D06
Locus At2g46740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19206964
Primers used for PCR KM19 - 8474, show primer details
   
Sequence Name 01-K033095-022-052-D06C-KM19
GenBank Accession LN486464 [GenBank]
Sequencing primer gene specific primer, KM19: 5'-ACATTTAGAAACGCCTCCATTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K033095-022-052-D06C-KM19
AAACATGGCATTTGGTCATTCTCCTTCCTATTGTTCGTTTTGGCGCACCCTTCTCGGGTT
ATATTGTTTATTTACACTTGTGCACACGGTAATCTCAACACCACCCGAAGATCCTGTTAA
ATGTGTGTCAGGAAACACGAACTGTATAGTCACCAACTCATTAGGAGCCTTCCCAGACCG
CTCCACGTGTAGAGCCGCCAACGTGGCTTACCCAACAACTGAAGCCGAGCTTGTATCTAT
CGTCGCTGCAGCTACTAAAGCCGGACGGAAAATGCGTGTAACCACTCGATATTCCCATAG
CATCCCTAAGCTCACGTGCACTGATGGAAACGACGGATTGTTCATTAGCACAAAGTTTCT
AAACCACACGGTGCAAGCCGATGCGAAGGCCATGACTTTGACAGTTGAGAGTGGCGTGAC
GCTNAGGCAATTAATCGCTGAAGCAGCTAAAGTTGGATTGGCGTTGCCTTATGCACCGTA
TTGGTGGGGAGTAACCTGGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
AT
   
Sequence Name 49-K033095-022-052-D06C-8409
GenBank Accession LN506743 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K033095-022-052-D06C-8409
CATTTCAGGTTACTCCCCACCAATACGGTGCATAAGGCAACGCCTATCCAACTTTAGCTG
CTTCAGCGATTAATTGCCTAAGCGTCACGCCACTCTCAACTGTCAAAGTCATGGCCTTCG
CATCGGCTTGCACCGTGTGGTTTAGAAACTTTGTGCTAATGAACAATCCGTCGTTTCCAT
CAGTGCACGTGAGCTTAGGGATGCTATGGGAATATCGAGTGGTTACACGCATTTTCCGTC
CGGCTTTAGTAGCTGCAGCGACGATAGATACAAGCTCGGCTTCAGTTGTTGGGTAAGCCA
CGTTGGCGGCTCTACACGTGGAGCGGTCTGGGAAGGCTCCTAATGAGTTGGTGACTATAC
AGTTCGTGTTTCCTGACACACATTTAACAGGATCTTCGGGTGGTGTTGAGATTACCGTGT
GCACAAGTGTAAATAAACAATATAACCCGAGAAGGGTGCGCCAAAACGAACAATAGGAAG
GAGAATAACCAAATGCCATGGTTTTTATTGAGTTTTGTCTAATGGAG

Last Updated on 06.12.2018 16:11