Sequences from confirmation amplicon


Confirmation sequences for line 052F01 and hit At1g70760 are shown.
Line ID 052F01
Locus At1g70760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26687358
Primers used for PCR MN32 - 8474, show primer details
   
Sequence Name 11-K031809-022-052-F01C-8409
GenBank Accession LN490996 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K031809-022-052-F01C-8409
GCCATTTACAATTAGCTAAGTGTACAACCAATCAACATGAATCTGCAGAAAACTCTCTGC
TTCTTCACCGTGACTTATTATTGTTCACTGCAGAATGTTAACAAGATGAGAGCAAAGGCC
TGTGATATACTTGGAGCAAAGAAGACAATCTTGGCAGCTCAACTCGGGGCAGTTCTTGCC
ACGGTAAGAACATACGAGACCTTTATTCACTATCGAAACTATGAGACAACAGTGGTCAGT
AATCGAAAATCGTTAGTTTTTCAGATTGACCATCCAGCCTTAGCAATAACAGGAGTTAAC
AACCAGCAGGAATTGAGCAGTGTTGTGCTCGATATCGGGATCATATCCGTTTGGTACTTC
CTAGTAATGCCAGTAAGTTTGAGGACACATACATTGTATTTTCAAAGATTTAGATGATTC
ATGATTATGAGTGTGGAGCTTATATATTATACTATGGTTTGTGTTAGCCAATCATCATGA
ACTGGCTAAGAGTAAGATGGTACAGAAGGAAGTTCTTCG
   
Sequence Name 12-K031809-022-052-F01C-MN32
GenBank Accession LN491455 [GenBank]
Sequencing primer gene specific primer, MN32: 5'-CGAAGAACTTCCTTCTGTACCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K031809-022-052-F01C-MN32
GATGATGGCTACACAAACCATAGTATAATATATAAGCTCCACACTCATAATCATGAATCA
TCTAAATCTTTGAAAATACAATGTATGTGTCCTCAAACTTACTGGCATTACTAGGAAGTA
CCAAACGGATATGATCCCGATATCGAGCACAACACTGCTCAATTCCTGCTGGTTGTTAAC
TCCTGTTATTGCTAAGGCTGGATGGTCAATCTGAAAAACTAACGATTTTCGATTACTGAC
CACTGTTGTCTCATAGTTTCGATAGTGAATAAAGGTCTCGTATGTTCTTACCGTGGCAAG
AACTGCCCCGAGTTGAGCTGCCAAGATTGTCTTCTTTGCTCCAAGTATATCACAGGCCTT
TGCTCTCATCTTGTTAACATTCTGCAGTGAACAATAATAAGTCACGGTGAAGAAGCAGAG
AGTTTTCTGCAGATTCATGTTGATTGGTTGTACACTTAGCTAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
G
Primers used for PCR MN33 - 8474, show primer details
   
Sequence Name 27-K031810-022-052-F01C-8409
GenBank Accession LN498265 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K031810-022-052-F01C-8409
GTATACAGAAAGGAGATTTGACACTGCGTGGCTTCAAGGAGAGAGATGGCAAAGCATTTG
GGGCATAGAGCCCAAGAGAGCCGCACCGGCTCATCTCTATGTGTCTGTTGTTCTTGTTTT
TGGTTGTGAAGTTTCCTTATCTCTTGAGATTGGTTCTAATGGCAGAGACTTCTAGGATTA
CTTTTGTCTGAATCACATAAATTCCACGTGTCATAAGGAGGTCCCACGTTTGATTTTGGC
ATATAGTGTTTTATTTCAAGAAAATGAAATAAAAACGAATAATCTCATTCCAAATTCCAC
ATGTATTCCAAATTTTATAGTTGCATCGCCGTTTCTGTAATTTGGTTCATGTCTTTGAAA
GTGAGGCAGTTCAAAGTAAGTAAATTGATGTAAATTAGAGTAGATATAACCAAAGAGAGA
TCAAGAATGGTGAAATGTGTCAATTGTCAAANNNNNAGAAAGAGTATTTACCACTTTTTT
TTCATTT
   
Sequence Name 28-K031810-022-052-F01C-MN33
GenBank Accession LN498726 [GenBank]
Sequencing primer gene specific primer, MN33: 5'-GACAATTGACACATTTCACCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K031810-022-052-F01C-MN33
TCTATTTACTCAATTTACTTACTTTGAACTGCCTCACTTTCAAAGACATGAACCAAATTA
CAGAAACGGCGATGCAACTATAAAATTTGGAATACATGTGGAATTTGGAATGAGATTATT
CGTTTTTATTTCATTTTCTTGAAATAAAACACTATATGCCAAAATCAAACGTGGGACCTC
CTTATGACACGTGGAATTTATGTGATTCAGACAAAAGTAATCCTAGAAGTCTCTGCCATT
AGAACCAATCTCAAGAGATAAGGAAACTTCACAACCAAAAACAAGAACAACAGACACATA
GAGATGAGCCGGTGCGGCTCTCTTGGGCTCTATGCCCCAAATGCTTTGCCATCTCTCTCC
TTGAAGCCACGCAGTGTCAAATCTCCTTTCTGTATTACATCTCACACCAGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11