Sequences from confirmation amplicon


Confirmation sequences for line 052G11 and hit At1g55710 are shown.
Line ID 052G11
Locus At1g55710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20826109
Primers used for PCR 67vi - 8474, show primer details
   
Sequence Name 82-K117997-0022-052-G11C-67vi
GenBank Accession LN514236 [GenBank]
Sequencing primer gene specific primer, 67vi: 5'-ATACTCATATGGTTCGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K117997-0022-052-G11C-67vi
GTGGAAGACTTGCAGACCACAAAATATTATTTCGGTGTTTAACAATCCCGAAAGCAACAC
AGAATGATATAAGTTTAAAAGGAGTTGTAAGAAACAAACCTTGAGGAACCGATGTTGATG
TTGTTCCTATGAAGAATCCTACCAGGAGCAACGGCACCACCATCGATCAACTCTCTAAGC
CCCCAATTCTCCACGTCCTCTTCTTTCACCTCCTTCTTCTTCTCCTTCTCCACATGGCGG
TTATTAGGAAGCTTCACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAAA
   
Sequence Name 42-K117996-0022-052-G11C-8409
GenBank Accession LN504628 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117996-0022-052-G11C-8409
ATTTACAATTGAATTATCCTGTGAAGCTTCCTAATAACCGCCATGTGGAGAAGGAGAAGA
AGAAGGAGGTGAAAGAAGAGGACGTGGAGAATTGGGGGCTTAGAGAGTTGATCGATGGTG
GTGCCGTTGCTCCTGGTAGGATTCTTCATAGGAACAACATCAACATCGGTTCCTCAAGGT
TTGTTTCTTACAACTCCTTTTAAACTTATATCATTCTGTGTTGCTTTCGGGATTGTTTAA
ACACCGAAATAATATTTTGTGGTCTGCTAAGTCTTTCCACATAATTAAATTGAAAGTAAA
GAGAAAAAAAGACAAACCACGAACCATATGAGTATAATGG

Last Updated on 06.12.2018 16:11