Sequences from confirmation amplicon


Confirmation sequences for line 052H04 and hit At5g23790 are shown.
Line ID 052H04
Locus At5g23790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8020212
Primers used for PCR 04xc - 3144, show primer details
   
Sequence Name 34-K119356-0022-052-H04C-04xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 04xc: 5'-CTGTTGAAAGTTTAGGTGCTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K119356-0022-052-H04C-04xc
GTTTTGGACCAAATCTTGTCCTTACGAGGATCTCCTTCGTGTTGTTCAAATCACCACTCC
AACTTATTTTGCCGAGCAGGTACGATTTTTTACGGATTTTCTATCATTTGATTTGACTTT
ATTATATTTTGTTACGGACTCTGATAATATTGTTTATTTTTGTTAGGACTTTCTGAATAT
ATACTTCAGAGACATTTACAAGCCAATCCCTTCGACCTACAACCTCGTAATGGCTATGCT
TTGGCGTCATCCCGAACACATTGACCTTGACCAAATCAGTGTTGTTCACTATTGTGCTAA
TGTTCGTTTTTTAAACTTTTTTTCTACAGATATGTAAATACATATTCTTATTACTAAAAC
CATTTGTATCTAAAATCAATTTTATAGGGTTCGAAGCCTTGGAAATTCGATGAAGCTGAA
GAGCATATGGATCGAGAAGACATAAAAATGTTGGTGAAGAAGTGGTGGGAGATTTACGAA
GATTCGAGTTTAGACTACAAGAATTTCGTCGAGACTGAGTCCAAGCTGAATCCGGTCACT
GCAACCTTGGCTTCCAAGAAACTTGTTGGCGATGTCCGGGAAACACTGATAGTTTAAACC
GAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACT
TCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTC
AGTGGAGATATC
   
Sequence Name 14-K119355-0022-052-H04C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K119355-0022-052-H04C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTCCCGGACATCG
CCAACAAGTTTCTTGGAAGCCAAGGTTGCAGTGACCGGATTCAGCTTGGACTCAGTCTCG
ACGAAATTCTTGTAGTCTAAACTCGAATCTTCGTAAATCTCCCACCACTTCTTCACCAAC
ATTTTTATGTCTTCTCGATCCATATGCTCTTCAGCTTCATCGAATTTCCAAGGCTTCGAA
CCCTATAAAATTGATTTTAGATACAAATGGTTTTAGTAATAAGAATATGTATTTACATAT
CTGTAGAAAAAAAGTTTAAAAAACGAACATTAGCACAATAGTGAACAACACTGATTTGGT
CAAGGTCAATGTGTTCGGGATGACGCCAAAGCATAGCCATTACGAGGTTGTAGGTCGAAG
GGATTGGCTTGTAAATGTCTCTGAAGTATATATTCAGAAAGTCCTAACAAAAATAAACAA
TATTATCAGAGTCCGTAACAAAATATAATAAAGTCAAATCAAATGATAGAAAATCCGTAA
AAAATCGTACCTGCTCGGCAAAATAAGTTGGAGTGGTGATTTGAACAACACGAAGGAGAT
CCTCGTAAGTGACAAGATTTGGTCCAAAAACCAACATACCGGCGTTGAAATATACCGGCG
GTGGAGCACCTAACTTTT

Last Updated on 06.12.2018 16:11