Sequences from confirmation amplicon


Confirmation sequences for line 053A02 and hit At4g21120 are shown.
Line ID 053A02
Locus At4g21120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11273230
Primers used for PCR RK11 - 3144, show primer details
   
Sequence Name 35-K016289-022-053-A02C-3144
GenBank Accession LN501619 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K016289-022-053-A02C-3144
AGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAA
ACTATCAGTGTCCGGCCATGCTGATATATTCAACCGAAGGGTACCCTGTTACGGAGATTT
TCTTTCTTTCATTTGTGAAGAATGCTATGAGAGCTGTCGCGGCAAGCATTACCACGGTCG
CGTTAATTGGTGTTCCGGTCTTTGCGTTGACTTGAGCTAGCCATGGAGGCATCATATGGG
CTCGGGCTATGTGTGTCATGTACCTAGCTTGACCAATGGCCCCAACTAACAAAACCGTCG
TCATACCTAAACATTTCAAAGATTAGTATAGATTTTAGACTATACTAAATGTAAACGCAA
ACGCAAATAAATAATG
   
Sequence Name 36-K016289-022-053-A02C-RK11
GenBank Accession LN502026 [GenBank]
Sequencing primer gene specific primer, RK11: 5'-TTTTGGCCTTCATTATTTATTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K016289-022-053-A02C-rk11
CGTTTCCTTTAGTATAGTCTAAAATCTATACTAATCTTTGAAATGTTTAGGTATGACGAC
GGTTTTGTTAGTTGGGGCCATTGGTCAAGCTAGGTACATGACACACATAGCCCGAGCCCA
TATGATGCCTCCATGGCTAGCTCAAGTCAACGCAAAGACCGGAACACCAATTAACGCGAC
CGTGGTAATGCTTGCCGCGACAGCTCTCATAGCATTCTTCACAAATGAAAGAAAGAAAAT
CTCCGTAACAGGGTACCCTTCGGTTGAATATATCAGCATGGCCGGACACTGATAGTTTAA
ACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGA
ACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTAC
GTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTT
TTCCACGATGCTCCTCGTGGGTGGGGGT
Primers used for PCR FI17 - 8474, show primer details
   
Sequence Name 14-K028095-022-053-A02C-8409
GenBank Accession LN492362 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K028095-022-053-A02C-8409
CCATTTCACAAAACTGAAAATTTTAGCCGATCTCCTTGTCTGTTTCCCCACTTTTCATCT
TCATGTTCGTTGCTGTGGCTCTTCTCGTTAGAAGATATTACGTCACCGGAGAAACATCGA
CCCGTGACCGTAACAAGTTTTTAGTGTTCTTAGGTTTGATTCTTGCGTCCTCTACCGCGA
CTGCTGTTTATTGGGCTTTGGAAGAAGAAGGTTGGATTGGGTATTGCATTACGGTTCCTA
TATGGTTTCTGTCTACGGTTGCCATGAAGTTTCTTGTACCGCAAGCTAGGGCTCCGAAAA
TTTGGGGTGTTCCTTTGGTTCCGTGGTTGCCTTCTGCTTCCATCGCTATTAATATCTTCC
TTCTTGGTTCCATCGATACAAAATCGTTCGTTAGGTTTGCAATCTGGACAGGTATTCTGC
TCATATATTACGTCTTGTTTGGGTTGCACGCAACGTATGATACAGCTAAGGCGACGTTGA
AGGAGAAGCAGGCATTGCAGAAAGCTGAGGAAGGTGGTGTTGTTGCTGATAATTCTTGTT
CT
   
Sequence Name 14-K028095-022-053-A02C-FI17
GenBank Accession LN492363 [GenBank]
Sequencing primer gene specific primer, FI17: 5'-TGAGCTTAAGTTGCAGAACAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K028095-022-053-A02C-FI17
GCTTTCTGCATGCCTGCTTCTCCTTCACGTCGCCTTAGCTGTATCATACGTTGCGTGCAA
CCCAAACAAGACGTAATATATGAGCAGAATACCTGTCCAGATTGCAAACCTAACGAACGA
TTTTGTATCGATGGAACCAAGAAGGAAGATATTAATAGCGATGGAAGCAGAAGGCAACCA
CGGAACCAAAGGAACACCCCAAATTTTCGGAGCCCTAGCTTGCGGTACAAGAAACTTCAT
GGCAACCGTAGACAGAAACCATATAGGAACCGTAATGCAATACCCAATCCAACCTTCTTC
TTCCAAAGCCCAATAAACAGCAGTCGCGGTAGAGGACGCAAGAATCAAACCTAAGAACAC
TAAAAACTTGTTACGGTCACGGGTCGATGTTTCTCCGGTGACGTAATATCTTCTAACGAG
AAGAGCCACAGCAACGAACATGAAGATGAAAAGTGTGGAAACAGACAAGAGATCGGCTAA
AATTTTCAGTTTTGTGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTAC

Last Updated on 06.12.2018 16:11