Sequences from confirmation amplicon


Confirmation sequences for line 053C02 and hit At1g76260 are shown.
Line ID 053C02
Locus At1g76260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28611322
Primers used for PCR 02cv - 8474, show primer details
   
Sequence Name 28-K109234-0022-053-C02C-02cv
GenBank Accession LN498884 [GenBank]
Sequencing primer gene specific primer, 02cv: 5'-TTTGGAGCACTGTGTAATTCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K109234-0022-053-C02C-02cv
TTAATTTTGCATACGAGAGAGAATCTTACGAGTATATGTTCTCTCTTGAGATTGTAGTCA
ACATTACGTACATGGGCACGTTCAATTGAATTATTCTTCCTGTAAATATGCAAACATATC
AAGCTCAACATAAAGGTATCTGATTTTGGAACCCAAAAATTAATAATGACAAACTATTGA
CTAGAGGAAACACGCTAGCTGTGCATGCAGGAATACAGTATATTAACTTTCATGCGAACA
TAAGAGAGGAACAAATATGACATACTTCATAGTACGTAAGTCCCAAAACTGGATAGATGA
TTCAGAAGTTGCTGCAACAGAATTCACATCATGTGGATTCCATGCTCCACCAGATAAAGA
ATGACGCATACCAGCTGACTCCTTAGATAAAACCTATAATTAGCAAAAAAGATGATTAAG
CGGAAAGTACCTCAAAGGTGGTCTAAAAACACAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 12-K109233-0022-053-C02C-8409
GenBank Accession LN491618 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K109233-0022-053-C02C-8409
TTGAATTATCCTGTGTTTTTAGACCACCTTTGAGGTACTTTCCGCTTAATCATCTTTTTT
GCTAATTATAGGTTTTATCTAAGGAGTCAGCTGGTATGCGTCATTCTTTATCTGGTGGAG
CATGGAATCCACATGATGTGAATTCTGTTGCAGCAACTTCTGAATCATCTATCCAGTTTT
GGGACTTACGTACTATGAAGTATGTCATATTTGTTCCTCTCTTATGTTCGCATGAAAGTT
AATATACTGTATTCCTGCATGCACAGCTAGCGTGTTTCCTCTAGTCAATAGTTTGTCATT
ATTAATTTTTGGGTTCCAAAATCAGATACCTTTATGTTGAGCTTGATATGTTTGCATATT
TACAGGAAGAATAATTCAATTGAACGTGCCCATGTACGTAATGTTGACTACAATCTCAAG
AGAGAACATATACTCGTAAGATTTCTCTCTCTGTATGCAAATATTTTAAATGCTTGTCAA
GCAATTCCAACAAAGAATTACACAGTGCTCCA

Last Updated on 06.12.2018 16:11