Genotyping and primer details


Genotyping and primer details for 053C02 - At1g76260
Line ID 053C02
Locus At1g76260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28611322 of line 053C02 Chr1:28611322
   
Insertion allele amplicon 8474 - 02cv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02cv: 5'-TTTGGAGCACTGTGTAATTCTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28611827
   
wt amplicon (over InsPos) 03dy - 02cv
wt amplicon start Chr1:28610840
wt amplicon end Chr1:28611827
wt amplicon size 988 bp
Locus specific primer 02cv: 5'-TTTGGAGCACTGTGTAATTCTTTG-3'
2nd locus specific primer 03dy: 5'-CGATTGAATGCATAAAGTTTCAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11